非小细胞肺癌中pokemon表达与p14^(ARF)、bcl-2的相关性及对预后的影响  

Correlation of the expression of pokemon with p14^(ARF) and bcl-2 and their effects on prognosis of non small cell lung cancer

作　　者：赵智宏[1] 王胜发[1] 丛德刚[1] 禹亮[1] 王巨[1] 

机构地区：[1]哈尔滨医科大学附属第一医院胸外科,哈尔滨150001

出　　处：《肿瘤》2008年第2期121-124,共4页Tumor

摘　　要：目的:探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中pokemon表达和p14ARF、bcl-2表达的关系及其对NSCLC临床病理特征和预后的判定价值。方法:应用SP免疫组织化学方法检测pokemon和p14ARF、bcl-2三种蛋白在62例NSCLC肿瘤组织及20例癌旁正常组织中的表达,分析它们与NSCLC病理学特征的关系,以及pokemon与p14ARF、bcl-2的相关性,并结合随访资料观察上述蛋白表达对NSCLC长期预后的影响。结果:正常肺组织中未见pokemon蛋白表达,p14ARF和bcl-2蛋白阳性表达率分别为95.0%和15.0%;在NSCLC组织中pokemon、p14ARF和bcl-2阳性表达率分别为72.6%、66.1%和53.2%。Pokemon表达与p14ARF表达呈负相关(r=-0.287,P<0.05),与bcl-2表达呈正相关(r=0.293,P<0.05)。Pokemon和p14ARF的表达与TNM分期相关(P<0.05),bcl-2表达与病理分型相关(P<0.05)。Pokemon与bcl-2表达阳性组的5年生存率分别为10.95%和13.74%,显著低于阴性组(P<0.05);p14ARF表达阳性组的5年生存率为21.68%,显著高于阴性组(P<0.05)。结论:NSCLC组织中存在pokemon和p14ARF、bcl-2的表达,pokemon表达与p14ARF表达呈负相关、与bcl-2表达呈正相关,它们对NSCLC的预后评估有一定的临床意义。Objective：To investigate the correlation of the expression of pokemon with p 14^ARF and bcl-2 in non small cell lung cancer （NSCLC） tissues, and their values for determining the clinicopathological characteristics and prognosis of NSCLC. Methods： Immunohistochemical SP staining was used to detect the expressions of pokemon, p 14^ARF, and bcl-2 proteins in 62 NSCLC tumor tissues and 20 adjacent normal tissues. Their relationship with the pathological characteristics of NSCLC was analyzed. The correlation of abnormal expressions of pokemon and p 14^ARF and bcl-2 was analyzed. The effects of the expressions of the three proteins on the long-term prognosis of NSCLC patients were observed based on the follow-up data. Results：In normal tissues there was no pokemon expression but the positive rates of p 14^ARF and bcl-2 expression were 95.0% and 15.0%, respectively. In NSCLC tissues, the positive rate!of pokemon, p 14^ARF,and bcl-2 protein were 72.6%, 66.1%, and 53.2%, respectively. The expression of pokemon had negative correlation with expression of p 14^ARF （r=-0.287, P〈0.05）, but had positive correlation with expression of bcl-2 （r=0.293, P〈0.05）. The expression of pokemon and p 14^ARF was associated with TNM staging （P〈0.05）. However, the expression of bcl-2 correlated with pathological classification （P〈0.05）. In pokemon and bcl-2 positive patients, the five-year survival rates were 10.95% and 13.74%, respectively, which were significantly lower than that in pokemon and bcl-2 negative patients （P〈0.05）. In contrast, the five-year survival rate was 21.68% in patients with positive expression of p 14^ARF, which was significantly higher than that in patients with negative expression of p 14^ARF （P〈0.05）. Conclusion：Pokemon, p 14^ARF, and bcl-2 are all expressed in NSCLC tissues. There is a negative correlation between the expression of pokemon and p 14^ARF and a positive correlation between the expression of pokemon and bcl-2, which has a certain clinical significance f

关 键 词：癌 非小细胞肺 基因表达 POKEMON P14^ARF BCL-2 免疫组织化学 预后 

分 类 号：R734.2[医药卫生—肿瘤]