Ezrin蛋白在肌病患者骨骼肌中的表达及意义  

Expression and significance of Ezrin in skeletal muscle of patients with myopathy

作　　者：赵清石[1] 江新梅[1] 胡静[2] 鞠浩[3] 袁军辉[2] 

机构地区：[1]吉林大学第一医院神经内科,长春130021 [2]河北医科大学第三医院神经肌病科 [3]北华大学附属医院神经内科

出　　处：《临床神经病学杂志》2008年第1期1-3,共3页Journal of Clinical Neurology

基　　金：国家自然科学基金资助项目(30340062);中华人民共和国人事部留学人员科技活动项目择优资助项目

摘　　要：目的研究Ezrin蛋白在肌病患者骨骼肌中的表达及意义。方法取肌纤维再生活跃的假肥大型肌营养不良(DMD,9例)和多发性肌炎(PM,5例)患者的骨骼肌标本,冰冻连续切片,进行HE染色及抗-Ezrin、抗-神经细胞黏附分子(NCAM)单克隆抗体免疫组化染色,观察被检肌的病理改变和Ezrin蛋白的表达。结果DMD、PM患者被检肌HE染色所见再生肌纤维直径较小、核位于中央、胞浆嗜碱性;NCAM染色再生肌纤维深染;再生肌纤维Ezrin呈阳性表达,伴随肌纤维成熟Ezrin表达逐渐减弱,成熟肌纤维无Ezrin表达;成肌细胞Ezrin呈阳性表达。结论Ezrin蛋白与DMD、PM肌病患者骨骼肌纤维再生可能存在密切关系。Objective To investigate the expression and significance of Ezrin in skeletal muscle of myopathy patients. Methods The skeletal muscle specimens with obviously regenerating muscle fibers were obtained from 9 patients with pseudohypertrophic muscular dystrophy （DMD） and 5 patients with polymyasitis （PM）. Then frozen serial sections were made and the sections were treated with HE stain, immunohistochemistry stains including anti-Ezrin and anti-Neural cell adhesion molecule （NCAM） monoclonal antibodies. The pathological changes and the expression of Ezrin were observed. Results The small regenerating fibers, central location of nucelus and basophilia endochylema were observed under HE stain beth in the patients with DMD and PM. Anachromaais of anti-NCAM monoclonal immunohistochemistry stain was found in the regenerating muscle fibers. Positive Ezrin expression was also detected in the regenerating fibers. However, this expression tapered gradually as the mature process of skeletal muscle. The expression of Ezrin was negative in the mature fibers. Ezrin expressed in the myoblasts. Conclusions Ezrin may be closely related to the regenerating skeletal muscle fibers of DMD and PM patients.

关 键 词：Ezfin蛋白 再生肌纤维 免疫组化染色 

分 类 号：R746[医药卫生—神经病学与精神病学]