多肿瘤标志物C12检测系统在胃癌诊断中的价值分析  

The Value of Tumor Marker Biochip Diagnostic System C12 in the Diagnosis and Monitor of Gastric Cancer

作　　者：杨雪琴[1] 陈创[1] 候晋轩[1] 杨国梁[1] 李雁[1] 

机构地区：[1]武汉大学中南医院肿瘤科武汉大学肿瘤防治研究中心

出　　处：《中国肿瘤临床》2008年第4期184-188,共5页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金创新群体(编号:20621502);国家自然科学基金(编号:20675058);全国优秀博士学位论文作者专项资金(编号:200464);武汉市创新研究课题基金资助(编号:20066002054)

摘　　要：目的:分析肿瘤标志物(tumormarkers,TM)诊断芯片C12在胃癌诊断中的价值。方法:分析总结156例胃癌初治患者12项TM的检测结果,寻找出与胃癌相关性最强的TM,计算各种TM组合方式对提高诊断率的作用。结果:C12诊断系统对本组胃癌患者的总体诊断率是35.90%,Ⅰ、Ⅱ、Ⅲ、Ⅳ期患者的诊断率分别是15.00%、25.00%、36.00%和47.72%,TM诊断率在各临床分期中的整体差异有统计学意义(χ2=7.81,P<0.05);单项TM诊断率最高者为CA19-9,诊断率为20.51%;诊断率最高的5种TM组合中,3种、4种、5种TM联合检测诊断率最高组合与CA19-9相比,差异有统计学意义(P<0.05);而2种、3种、4种、5种TM联合检测诊断率最高的组合与12项联合检测之间的整体差异无统计学意义(χ2=1.193,P>0.05)。结论:C12系统对诊断晚期胃癌有一定价值,但用于早期胃癌的灵敏度不高。Objective： To explore the diagnostic value of tumor marker （TM） hioehip diagnostic system C12 in the diagnosis and monitoring of gastric eaneer. Methods： Using the C12 diagnostic biochip system, we screened the serum of 156 pathologically confirmed gastric cancer patients for 12 TMs including carcinoembryonic antigen（CEA）, alpha-fetoprotein （AFP）, carbohydrate antigen 19-9（CA19-9）, carbohydrate antigen 242（CA242）, cancer antigen 15-3（CAI5-3）, cancer antigen 125 （CA 125）, prostate specific antigen （PSA）, free-PSA, neuron-speeifie enolase （NSE）, human chorionie gonadotropin-beta（β-HCG）, human growth hormone（HGH）, and ferritin（Fe）. The results were compared among different clinical stages of gastric cancer. Results： The overall sensitivity in these 156 patients of the C12 bioehip system was 35.90%. The diagnostic tale of the C12 biochip system was 15.00% in stage Ⅰ patients, 25.00% in stage Ⅱ palients, 36.00% in stage Ⅲ patients, and 47.73% in stage Ⅳ patients, with a statistical significance （contingency table chi-square lest, χ^2= 7.81. P〈0.05）. Among the 12 TMs, CA19-9 had the highest sensitivity of up to 20.51%, With a diagnostic rote of 33.33%, the combined detection of CA19-9, CEA, CA125 and AFP was the most beneficial combination. Conclusion： The C12 biochip diagnostic system is valuable tot the diagnosis of gastric cancer, but its sensitivity for early stage gastric cancer is not satisfactory. Optimizing these TMs and developing new and more effective biochip diagnostic systems are urgent.

关 键 词：胃癌 肿瘤标志物 临床分期 早期诊断 蛋白芯片 

分 类 号：R735.2[医药卫生—肿瘤]