X射线对肺腺癌细胞粘附分子及其相关生物学特性的影响  

The effect of X-irradiation on adhesion molecules and their related biological characteristics of A549 human lung adenocarcinoma cell line

作　　者：刘晓[1] 蒋幼凡[1] 岳秀[1] 雷淑慧[1] 

机构地区：[1]重庆医科大学附属第二医院呼吸内科

出　　处：《重庆医科大学学报》2008年第1期25-28,共4页Journal of Chongqing Medical University

基　　金：重庆市卫生科技项目(03-2-053)

摘　　要：目的:探讨X射线对肺腺癌细胞粘附分子及其相关生物学特性的影响。方法:不同剂量X射线照射肺腺癌细胞A549,MTT法检测细胞增殖抑制;免疫细胞化学法蛋白水平检测细胞中E-cadherin及其连环蛋白β-catenin的表达;Transwell小室检测细胞的侵袭力。结果:X射线对A549细胞的增殖抑制作用呈剂量依赖性;照射后E-cadherin和β-catenin蛋白的表达均显著高于对照组(P<0.05),其变化趋势是随照射剂量增加表达逐渐增加;Transwell小室结果表明随着照射剂量的增加,细胞侵袭力逐渐降低(P<0.05)。结论:X射线上调A549细胞上粘附分子E-cadherin和β-catenin在细胞中的表达,增加细胞间的粘附力,降低肿瘤细胞的侵袭力,这可能是X射线抑制肺腺癌细胞转移的作用机制之一。Objective：To investigate the effect of X-irradiation on Adhesion molecules and their related biological characteristics of Human Lung. Methods：AS49 cells were irradiated with varying doses of X-rays. MTr assay was employed to evaluate the effects of X-irradiation on the proliferative inhibition of A549 cells. The immunocytochemical method was employed to analyze the protein expression levels of E-cadherin and β-catenin. The transwell chambers were employed to evaluate the invasion of A549 cells. Results：X-irradiation could inhibit the proliferation of A549 cells in a dose-dependent manner. The protein expression of E-cadherin and β-catenin in A549 were higher than that in control group（P〈0.05）.And the cell invasions were lower than that in control group （P〈0.05）. The expression of E-cadherin, β-catenin and cell invasions showed a direct correlation with the dose. Conclusion：Up-regulating the expression of E-cadherin and β- catenin on A549 cells,which enhanced the adhesion between cells and depressed the invasion. That may be the mechanism of X-irradiation inhibiting the metastasis of Human Lung Adenocarcinoma Cell line.

关 键 词：X射线 A549 E-CADHERIN Β-CATENIN 转移 

分 类 号：R734.2[医药卫生—肿瘤] R730.55[医药卫生—临床医学]