金黄地鼠颊囊黏膜癌变过程中iNOS的表达与Bcl-2的关系  

Correlation Between Nitric Oxide Synthase and Bcl-2 Expressions in Hamster Buccal Pouch Carcinogenesis

作　　者：张晓英[1] 孙善珍[2] 马伯龙[2] 王霞[2] 张春艳[2] 

机构地区：[1]山东大学齐鲁医院口腔内科,山东济南250012 [2]山东大学口腔医学院组织病理研究室,山东济南250012

出　　处：《口腔颌面外科杂志》2008年第1期15-18,共4页Journal of Oral and Maxillofacial Surgery

基　　金：山东省科技厅资助课题(2002BB1CAA1)

摘　　要：目的:观察金黄地鼠颊囊黏膜癌变过程中诱导性一氧化氮(inducible nitric oxide synthase,iNOS)的表达与凋亡相关蛋白Bcl-2的关系。方法:6～8周龄金黄地鼠72只,随机分为实验组(60只)和空白对照组(12只),空白对照组地鼠不做任何处理,实验组地鼠用0.5%的二甲基笨丙蒽(dimethyl-benzanthrance,DMBA)丙酮溶液诱导生成颊囊黏膜癌,并在黏膜癌变的不同阶段检测组织中iNOS和Bcl-2的表达。结果:正常地鼠颊囊黏膜组织中未见iNOS阳性表达,组织中iNOS、Bcl-2的表达强度在颊囊黏膜癌变过程中均呈上升趋势。鳞癌组织中iNOS的表达较单纯增生组织显著增强(P<0.01);鳞癌组织中Bcl-2的表达强度明显高于轻度上皮异常增生组(P<0.05),但与重度和中度上皮异常增生组之间无显著性差异(P>0.05);Bcl-2的表达强度随iNOS表达强度的增强呈现先升后降的复杂变化。结论:一氧化氮参与了颊囊黏膜鳞癌的发生、发展,并可能通过Bcl-2途径调节肿瘤细胞的凋亡。Objective： To observe the correlation of inducible nitric oxide synthase （iNOS） with Bcl-2 in the golden hamster buccal pouch carcinogenesis induced by dimethyl-benzanthrance （DMBA）. Methods： 72 outbred six-week old Syrian golden hamsters were randomly divided into experimental group （60 animals） and control group（12 animals）. The pouches of the experimental hamsters were painted with 0.5% DMBA solution three times a week. Light microscope was used to observe the pathologic changes. Expressions of iNOS and Bcl-2 were observed by immunohistochemistry. Results： In oral carcinoma group iNOS and Bcl-2 protein expression were significantly increased compared with the normal or hyperplasia group（P〈0.05）. There was complicated relationship between nitric oxide and Bcl-2. Conclusion： Nitric oxide might suppress or induce cell apoptosis by regulating apoptotic signaling cascades in Bcl-2.

关 键 词：金黄地鼠 一氧化氮 BCL-2 颊囊 

分 类 号：R739.8[医药卫生—肿瘤]