Bcl-2基因家族相关蛋白在人卵巢癌耐药细胞凋亡中的作用  

Effect of bcl-2 Gene Family Proteins in Apoptosis of Human Ovarian Carcinoma Multidrug Resistant Cells

作　　者：李佳平[1] 周洪贵[1] 唐良萏[2] 卞度宏[2] 

机构地区：[1]川北医学院附属医院,四川南充637000 [2]重庆医科大学第一医院,重庆400016

出　　处：《肿瘤学杂志》2008年第2期118-120,共3页Journal of Chinese Oncology

基　　金：四川省卫生厅基金项目(070306)

摘　　要：[目的]探讨bcl-2基因家族相关蛋白在卵巢癌耐药细胞SKOV3/ADM发生凋亡中的作用。[方法]用免疫细胞化学法测定SKOV3和SKOV3/ADM细胞的bcl-2蛋白;以不同浓度的阿霉素作用于SKOV3/ADM细胞,用流式细胞仪动态分析细胞周期和凋亡,并在不同时间点观察其bcl-2、bax、bad和p21蛋白变化。[结果]SKOV3/ADM细胞的bcl-2蛋白显著高于SKOV3(P<0.01);大剂量阿霉素(10μg/ml和40μg/ml)作用3h后,能明显下调SKOV3/ADM的bcl-2蛋白和增加bad蛋白;小剂量(0.5μg/ml)阿霉素作用(24、48、72h)后,SKOV3/ADM细胞的凋亡发生率(10.08%、30.53%和33.7%),显著低于SKOV3(40.01%、65.38%和77.65%)(P<0.01);SKOV3/ADM细胞p21、bad蛋白增加,bcl-2蛋白下降。[结论]SKOV3/ADM细胞bcl-2高表达能拮抗化疗剂诱导凋亡的作用,bcl-2基因家族相关蛋白与卵巢癌耐药细胞凋亡密切相关。[Purpose] To investigate the effect of bcl-2 gene family proteins in apoptosis of human ovarian carcinoma multidrug resistant（MDR） cells SKOV3/ADM. [Methods] Immunocytochemistry was used to detect bcl-2 protein expression in ovarian carcinoma SKOV3 ceils and its MDR cells SKOV3/ADM. Ceil cycle and apoptosis were measured by flow cytometry, and protein bcl-2, bad, bak and p21 were detected on different time sport in SKOV3/ADM cells which were treated by adriamycin with various concentrations. [Results] Bcl-2 expression were higher in SKOV3/ADM cells than that in SKOV3 ceils （P〈0.01）. Bcl-2 was downregnlated and bad was upregnlated in SKOV3/ADM ceils treated with adriamycin 10μg/ml and 40μg/ml for 3h. After incubated with adriamycin 0.5μg/ml for 24,48,72h, apoptosis percentage in SKOV3/ADM cells was significantly lower than that in SKOV3 ceils （10.08%, 30.53% and 33.7% vs 40.01%, 65.38% and 77.65%, respoctivdy, P〈0.05）, the expression of p21 and bad increased and the expression of bcl-2 decreased significantly. [Conclusion] Overexpression of bcl-2 in SKOV3/ADM ceils could inhibit apoptosis induced by chemotherapeutic agents. The bcl-2 gene family proteins significantly associated with apoptosis in human ovarian carcinoma multidrug resistant （MDR） cells.

关 键 词：卵巢肿瘤 细胞凋亡 bcl-2 BAD BAX 

分 类 号：R737.31[医药卫生—肿瘤]