适合微创研究的山羊腰椎间盘突出模型的建立  

A novel sheep model of intervertebral disk displacement for intradiscal therapy study

作　　者：商卫林[1] 李振宙[1] 侯树勋[1] 吴闻文[1] 刘宁[2] 李韶然[3] 吴叶[1] 

机构地区：[1]解放军总医院第一附属医院骨科,北京100037 [2]解放军总医院第一附属医院放射科 [3]解放军总医院第一附属医院病理科

出　　处：《中国骨肿瘤骨病》2008年第1期1-5,58,共6页Chinse Journal Of Bone Tumor And Bone Disease

摘　　要：目的建立用于微创椎间盘内治疗研究的山羊腰椎间盘突出模型。方法9 只6月龄山羊,随机分成3组,每组18个腰椎间盘,将每只山羊的6个腰椎间盘随机等分成正常组和模型组。模型组椎间盘左前外侧纤维环用直径2.5mm的微型环锯限深5mm造成柱状纤维环缺损。术后2、4、6个月时对正常及模型椎间盘观察X线片上的椎间盘高度指数、MRIT2加权相上的椎间盘高信号区的面积和平均T2弛豫时间、椎间盘纤维环损伤区的形态学变化、组织病理切片(HE染色、Masson三色胶原染色和番红O-固绿染色)椎间盘组织形态学变化、生物化学变化和组织学评分等指标。结果正常成年山羊椎间盘组织学结构特点与人类相似;模型椎间盘在损伤后2个月即出现明显退变(P<0.01),包括椎间盘高度指数降低、椎间盘T2加权高信号区面积和平均T2弛豫时间减低、组织学退变评分增高;MRI和组织学均发现损伤纤维环外侧1/3修复,内2/3出现髓核组织突出充填。损伤后4、6 个月模型椎间盘退变加重,但与损伤后2个月的指标无显著性差异(P>0.05);椎间盘突出形态稳定。结论以成年山羊为对象,应用微型环锯对椎间盘前外侧纤维环定量损伤可以获得快速、有效、重复性好的腰椎间盘突出动物模型。Objective To develop a novel sheep model of intervertebral disk displacement for studying intradiscal therapy. Methods 9 adult sheep were randomly divided into 3 groups according to the time to be sacrificed, 6 intervertebral disks of each sheep were randomly and equally divided into control group and model group. Left anterolateral annulus fibrosus of intervertebral disks in model group were partial resected with 2.5ram-diameter-hole saw in depth of 5mm. Disk height index of intervertebral disks on X-ray films, areas and average T2 relaxation time of high intensity zone of disks on T2-weight MRI and histological score on pathological sections （HE staining, Masson staining and safranin O-fast green staining） were measured at 2 months, 4 months and 6 months after operation. The morphology of intervertebral disk displacement at the injured sites was also observed on axial T2-weight MRI and pathological sections. Results The structure and histology of sheep is similar to humans. Disk height index of model intervertebral disks on X-ray films, areas and average T2 relaxation time of high intensity zone of model disks on T2-weight MRI decreased significantly （P〈0.01, vs control）; histological score of model disks increased significantly （P〈0.01, vs control） at 2 months, 4 months and 6 months after operation. Abovementioned indices aggravated gradually during 2-6 months after operation, but had no significant difference among 2 months, 4 months and 6 months after operation. The outermost 1/3 annulus defect showed the ability to heal, the defect induced by the hole saw led initially to deformation and bulging of the collagen bundles, eventually to inner extension of the tear and complete failure, nucleus pulposus protruded into the inner 2/3 defect. Conclusion A novel sheep model of intervertebral disk displacement can be developed by partial resection of anterolateral annulus fibrosus with 2.5mm-diameter-hole saw in depth of 5mm quantitatively, which is simple, rapid, effective and reproducible.

关 键 词：疾病模型 动物 椎间盘移位 椎间盘退变 山羊 组织学 

分 类 号：R-332[医药卫生] R681.53