相同遗传背景鼻咽癌细胞株辐射抗拒与STR基因座相关性的研究  

Relationship between radioresistance and STR loci in the nasopharyngeal carcinoma cells with same genetic background

作　　者：王旭丹[1] 曲昌菊[1] 杨惠玲[1] 粱志慧 郭禹标[3] 郑芹[1] 

机构地区：[1]中山大学中山医学院病理生理学教研室,广东广州510089 [2]中山大学肿瘤医院,广东广州510060 [3]中山大学附属第一医院内科

出　　处：《国际内科学杂志》2008年第2期71-73,共3页International Journal of Internal Medicine

基　　金：国家自然科学基金(30670627);广东省自然科学基金(06021210);广东重大科技专项基金(30604);广东省重点科技攻关项目(2004A30801006);广州市科技计划(国际合作)项目(2004J1-10041)

摘　　要：目的在验证鼻咽癌细胞CNE-2和CNE-2R不同辐射抗拒性的基础上,探索STR基因座在CNE-2和CNE-2R中的表达差异。方法快速Chelex-100法提取CNE-2和CNE-2R细胞株的DNA,利用Pow-erPlex(16体系荧光标记的多重聚合酶链反应(PCR)系统对15个常染色体STR基因座(D3S1358,TH01,D18S51,PentaE,D5S818,D13S317,D7S820,D16S539,CSF1PO,Penta D,Vwa,D8S1179,TPOX和FGA)和性别基因(Amelogenin)进行扩增,并在ABI3100型遗传分析仪上对PCR扩增产物进行电泳分析,最后用GeneMapper(3.0软件对等位基因进行自动分型。结果本研究发现在检测的15个STR基因座和性别基因Amelogenin中,CNE-2和CNE-2R细胞STR基因座的表达存在差异,即与CNE-2细胞相比,CNE-2R细胞D8S1179,D13S317和TH01 3个基因座的表达存在显著差异,其中D8S1179基因座存在等位基因的缺失;而D13S317和TH01 2个基因座等位基因间的峰高比值和峰面积比值存在显著差异。结论不同辐射抗拒鼻咽癌细胞株CNE-2和CNE-2R细胞的STR基因座表达存在量和质的差异,提示变异的STR基因座可能参与鼻咽癌细胞CNE-2辐射抗拒的诱导。Objective To discuss the discrepancy of STR loci in nasopharyngeal carcinoma（ NPC ） cells CNE- 2 and CNE-2R on the basis of validating their different radioresistanee. Methods DNA samples from CNE-2 and CNE-2R were extracted with rapid Chelex-100. Fifteen STR loci （ D3S1358, TH01, D18S51, PentaE, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, Vwa, D8Sl179,TPOX, FGA） and a sex gene （Amelogenin） were amplified with fluorescent labeled multiplex PCR system. The PCR products were analyzed by ABI3100 and the alleles were typed with GeneMapper 3.0. Results Compared with CNE-2 cells, the D8S1179 locus in CNE-2R cells presents allelie deficiency , while the D13S317 and TH01 loci present distinct discrepancy of peak height ratio and peak area ratio. Discussion The discrepancy of STR loci is present in different radioresistant NPC cell lines and maybe related with radioresistanee.

关 键 词：鼻咽肿瘤 肿瘤细胞 培养的 STR基因座 辐射抗拒 

分 类 号：R739.6[医药卫生—肿瘤]