成釉细胞瘤中细胞周期素D1及其抑制因子的表达  

Expression of cyclin D1 and its inhibitors in ameloblastomas

作　　者：薛明[1] 钟鸣[1] 王洁[1] 张波[2] 侯琳[2] 

机构地区：[1]中国医科大学口腔医学院病理科,沈阳110002 [2]北京大学医学部病理系

出　　处：《现代口腔医学杂志》2008年第2期164-166,共3页Journal of Modern Stomatology

基　　金：辽宁省自然科学基金(20032057);辽宁省教育厅基金(2012217);沈阳市科委基金(200138-7)

摘　　要：目的研究细胞周期素D1(cyclin D1)、周期素依赖激酶抑制因子p16INK4、p21WAF1mRNA和p27KIP1蛋白在人成釉细胞瘤(ameloblastomas,ABs)中的表达及其临床生物学意义。方法用原位杂交和免疫组化(SP法),分别检测ABs中cyclin D1、p16INK4、p21WAF1mRNA和p27KIP1蛋白的表达。结果cyclin D1mRNA阳性率为42.6%(23/54);p16INK4、p21WAF1mRNA和p27KIP1蛋白的阳性率分别为31.5%(17/54)、22.6%(12/53)、16.7%(9/54)。伴随ABs的复发与恶变cyclin D1mRNA阳性率逐渐上升,p16INK4、p21WAF1mRNA和p27KIP1蛋白阳性表达丢失。经Kendall相关性分析得出,cyclin D1mRNA与p21WAF1mRNA、p27KIP1蛋白之间在AB中rk分别为-0.213、-0.284,均P<0.05。结论AB的发生发展可能与cyclin D1过表达及p16INK4、p21WAF1mRNA、p27KIP1蛋白丢失等多种因子共同调控有关。Objective To investigate the expressions of eyelin D1 mRNA, and eyelin - dependment kinase inhibitors p16^INK4, p21^WAF1 mRNA, and p27^KIP1 protein in human ameloblastoma. Methods The expression of eyelin D1,p16^INK4, p21^WAF1 mRNA, and p27^KIP1 protein in 54 eases of human ameloblastoma were detected by in situ hybridization and immunohistochemy （SP method）, respectively. Results The positive ratio of eyelin D1 mRNA, p16^INK4, p21^WAF1 mRNA, and p27^KIP1 protein was42.6% （23/54）, 31.5% （17/54）, 22.6% （12/53）, and 16.7% （9/54）, respectively. In recurred and malignantly transformed ameloblastoma, the expression of eyelin DI mRNA increased, while the mRNA expression of p16^INK4 and p21^WAF1, and protein expression of p27^KIP1 decreased or lost. The expression of eyelin D1 mRNA was negatively correlated with the mRNA expression of p16^INK4 and p21^WAF1 and protein expression of p27^KIP1 in ameloblastoma （rk = -0. 213, rk = -0.284, P 〈 0.05 ）. Conclusion The development of AB may be related to the high expression of evelin D1 and loss of p16^INK4 and p21^WAF1 mRNA. and p27^KIP1 protein.

关 键 词：成釉细胞瘤 细胞周期素D1 P16^INK4 P21^WAF1 P27^KIP1 

分 类 号：R739.8[医药卫生—肿瘤]