双(α-呋喃甲酸)氧钒的胰岛素样作用  

Insulino-mimetic effects of bis (α-furancarboxylato) oxovanadium (IV) in vitro

作　　者：李艳蓉[1] 高丽辉[1] 马燕琳[1] 牛艳芬[1] 刘伟平[2] 李玲[1] 

机构地区：[1]昆明医学院云南省天然药物药理重点实验室,云南昆明650031 [2]昆明贵金属研究所,云南昆明650221

出　　处：《药学学报》2008年第3期318-322,共5页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(30260118)

摘　　要：To study insulino-mimetic effects of bis（α-furancarboxylato）oxovanadium（IV）（BFOV）,a orally active antidiabetic vanadyl complex,on glucose uptake and lipogenesis in isolated rat adipocytes were determined by using 2-deoxy-D——glucose and D-glucose,respectively.Lipolysis was assayed by free fatty acids（FFA） released from isolated rat adipocytes treated with epinephrine.The results showed that BFOV,similar to insulin,concentration-dependently significantly enhanced the uptake of 2-deoxy-D——glucose and the transformation from D-glucose to lipid in isolated rat adipocytes,with the EC50 values of（0.31±0.08） mmol·L-1 and（0.49±0.12） mmol·L-1,respectively.Moreover,BFOV markedly inhibited FFA release from isolated rat adipocytes treated with epinephrine,and the IC50 value was（0.30±0.20） mmol·L-1.BFOV had insulino-mimetic effects such as enhancing glucose uptake and lipogenesis,as well as inhibiting lipolysis.To study insulino-mimetic effects of bis （α-iurancarboxylato） oxovanadium （ Ⅳ） （ BFOV） , a orally active antidiabetic vanadyl complex, on glucose uptake and lipogenesis in isolated rat adipocytes were determined by using 2-deoxy-D-[3H ]-glucose and D-[3H ]-glucose, respectively. Lipolysis was assayed by free fatty acids （FFA） released from isolated rat adipocytes treated with epinephrine. The results showed that BFOV, similar to insulin, concentration-dependently significantly enhanced the uptake of 2-deoxy-D-[ 3H ]-glucose and the transformation from D-[ 3H ]-glucose to lipid in isolated rat adipocytes, with the EC50 values of （0.31 ＋ 0. 08） mmol · L^-1 and （0.49 ＋ 0. 12） mmol· L^-1, respectively. Moreover, BFOV markedly inhibited FFA release from isolated rat adipocytes treated with epinephrine, and the IC50 value was （0. 30 〉 0. 20） mmol · L^-1 BFOV had insulino-mimetic effects such as enhancing glucose uptake and lipogenesis, as well as inhibiting lipolysis.

关 键 词：双(Α-呋喃甲酸)氧钒 脂肪细胞 葡萄糖摄取 脂肪生成 脂肪分解 

分 类 号：R965[医药卫生—药理学] R977.15[医药卫生—药学]