咪唑克生干预对EAE脊髓免疫细胞及IFN-γ与IL-10水平的影响  

Intervention Effect of Idazoxan on Immunologic-reactive Cells and IFN-γ,IL-10 in the Spinal Cord of EAE

作　　者：朱振国[1] 郑荣远[1] 陈国钱[1] 韩钊[1] 邱伟文[1] 殷为勇[1] 

机构地区：[1]温州医学院附属第一医院神经内科,浙江温州325000

出　　处：《中国神经免疫学和神经病学杂志》2008年第2期97-99,131,I0002,共5页Chinese Journal of Neuroimmunology and Neurology

基　　金：国家自然基金资助项目(30470611);浙江省自然基金资助项目(Y30470611)

摘　　要：目的探讨咪唑克生(Ida)对Lewis大鼠实验性自身免疫性脑脊髓炎(EAE)的中枢神经系统(CNS)炎症的影响。方法实验分Ida按体重16 mg/kg2、mg/kg两组和对照组。用豚鼠脊髓匀浆(GPSCH)诱发Lewis大鼠EAE,并于给予GPSCH当日开始分别腹腔注射Ida(Ida组)和生理盐水(对照组)连续10 d,观察EAE症状并评分。第15天处死动物,用免疫组化法测定腰髓中表达胶质纤维酸性蛋白(GFAP)和诱导型一氧化氮合酶(iNOS)的阳性细胞数;用ELISA法测定颈髓IFN-γ和IL-10含量。结果按体重2 mg/kg Ida能促进腰髓星形胶质细胞增殖活化,使表达iNOS的炎症细胞减少,并能增加颈髓中IL-10的含量,与对照组比较差异均有统计学意义(均P<0.05)。而按体重16 mg/kg Ida也有类似作用,但与对照组比较差异无统计学意义。结论按体重2 mg/kg Ida对Lewis大鼠EAE炎症具抑制作用。Objective To explore the anti-inflammatory effects of idazoxan on the experimental autoimmunity encephalomyelitis（EAE） in Lewis rats. Methods Homogenate of the fresh spinal cord from guinea pigs （GPSCH） in saline with an equal volume of complete freund＇s adjuvant（CFA） was injected into Lewis rats as antigen to induce the model of NAN. Meanwhile, Idazoxan was injected intraperitoneally at 0 - 10 days after successive immunization. All Lewis rats were divided into 16 mg/kg idazoxan group,2 mg/kg idazoxan group and control （model）group, the scores of symptoms were observated. On the 15th day after immunization, all lewis rats were killed and perfused with saline. Cells those expressed glial fibrillary acidic protein（GFAP） and inducible nitricoxide synthase（iNOS） were counted with immunohistochemistry. The concentration of IFN-γ and IL-10 were measured with enzyme-linked immunosorbent assay（ELISA）. Results 2 mg/kg idazoxan could proliferate and activate stroeytes in the lumbar cord （P〈 0. 05）, and reduce number of inflammatory cells with iNOS expressed（P〈 0. 05）. 16 mg/kg idazoxan also had the similar effect, but had no statistically significance comparing with the control group. Conclusions 2 mg/kg idazoxan might have anti-inflammatory effects on EAE in Lewis rats.

关 键 词：实验性脑脊髓炎 咪唑克生 干扰素-Γ 白介素-10 

分 类 号：R744.5[医药卫生—神经病学与精神病学]