P27和CyclinE在胆囊癌组织中的表达及意义  

EXPRESSION AND SIGNIFICANCE OF P27 AND CYCLINE IN GALLBLADDER CARCINOMA

作　　者：姚坦[1] 孟翔凌[2] 

机构地区：[1]安徽省六安市人民医院,六安237000 [2]安徽医科大学第一附属医院

出　　处：《肝胆外科杂志》2008年第1期41-44,共4页Journal of Hepatobiliary Surgery

摘　　要：目的探讨P27和CyclinE的表达在胆囊癌组织中的表达及其意义。方法采用免疫组化SP法检测P27和CyclinE的表达状况,并以同期5例慢性胆囊炎组织作为对照。结果(1)慢性胆囊炎组织中P27的高表达阳性率为100%(20/20),明显高于胆囊癌的45%(18/40),(P<0.05)。CyclinE在胆囊癌组织中表达阳性率为67.5%(27/40),显著高于慢性胆囊炎的15.0%(3/20),(P<0.05)。(2)P27的表达与胆囊癌组织学分化程度、Nevin分期、肿瘤浸润深度、淋巴结转移、周围浸润有关(P<0.05)。CyclinE的表达与胆囊癌组织学分化程度、Nevin分期进展、肿瘤浸润深度有关(P<0.05)。P27和CyclinE的表达与胆囊癌患者的预后明显相关(P<0.05)。(3)P27和CyclinE表达之间呈负相关关系(r=-0.375,P<0.05)。结论P27表达下降和CyclinE阳性表达导致细胞周期的调控异常,可能参与了胆囊癌的发生、发展过程。Objective To explore the expression of CyclinE and P27 protein and their significance in gallbladder carcinoma. Methods The expression of CyclinE, P27 in gallbladder carcinoma were detected by immunohistochemical staining technique （SP method）, 15 cases chronic cholecystitis tissues were collected as contrast. Results （ 1 ） The positive expression rate of p27 in gallbladder carcinoma was 45.0% （ 18/40）, which was lower than that in chronic cholecystitis（ 100% ）, P 〈0.05. The positive expression rate of cyclinE in gallbladder carcinoma was 67.5% （27/40）, which was significantly higher than that in chronic cholecystitis （ 15.0% ,3/20）, P 〈 0. 05. （2）The expression level of p27 protein in gallbladder carcinomas showed an obvious difference in the histological grade, nevins stage, depth of invasion, lymph node metastasis, infiltration （P 〈 0. 05）. The expression level of cyclinE protein in gallbladder carcinomas showed an obvious difference in the histological grade, nevins stage, depth of invasion （P 〈 0. 05）. Log rank test showed that the p27 and cyclinE expression were significantly related with prognosis of the patients （ P 〈 0.05 ）. （ 3 ） There was negative correlation between p27 and cyclinE expression （ r = - 0. 375, P 〈 0. 05 ）. Conclusion The decreased expression of p27 and the high expression of cyclin E result in abnomal regulation of cell cycle,which may be associated with gallbladder carcinogenesis and progression.

关 键 词：P27 CyclinE胆囊癌 免疫组织化学 

分 类 号：R735.8[医药卫生—肿瘤]