麻黄内生真菌的分离及其生物活性初步研究  

Study on Bioactivity of Endophytic Fungi from Ephedra

作　　者：黄丹虹[1] 阎雪芬[1] 黄耀坚[1] 张连茹[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2008年第2期248-252,共5页Journal of Xiamen University：Natural Science

基　　金：福建省自然科学基金(2006J0378)资助

摘　　要：从内蒙古赤峰等地的中草药麻黄中分离得到141株内生真菌,体外抗氧化、抗肿瘤等活性测定表明,有11株内生真菌对H2O2具有较好的清除活性(LD50均大于50,LD50为抑制率为50%时的样品稀释倍数),占菌株总数的7.80%;有27株内生真菌对Raji和HepG-2具有肿瘤细胞毒活性(LD50大于50),占菌株总数的19.15%,其中以抑制悬浮Raji细胞为主.此外,在141株菌中,有74株对一种或二种以上的指示菌显示出抑菌活性,占菌株总数的52.48%.本实验结果表明,麻黄中含有丰富的内生真菌,是抗氧化、抗菌及抗肿瘤等活性物质的重要来源.为进一步从中草药植物中分离筛选新药先导化合物提供了科学依据.One hundred and forty-one endophytic fungi strains were isolated from ephedra that were from Chifeng et al places in Inner Mongolia,and their bioactivities were primarily studied. Results indicated： all of the samples,eleven fungi strains had the ability of scavenging H2O2 （with the LD50 above 50）, accounting for 7.80% of the strains tested, twenty-one fungi strains with high cytotoxic activity（with the LD50 above 50） were screened out,accounting for 19.15 % of the total strains,and this ability focused on inhibition of the growth of Raji cells,accounting for 17.73% of all the strains. Furthermore,seventy-four fungi strains had antimicrobial activities against one or more microbial,accounting for 52.48% of the strains tested. This study could provide scientific knowledge of screening new-leading compounds of medicine from Chinese herbs.

关 键 词：麻黄 内生真菌 抗菌活性 抗氧化活性 抗肿瘤活性 

分 类 号：Q939.92[生物学—微生物学]