脂多糖受体CD14通过核因子-κB途径参与大鼠急性肝功能衰竭的动态观察  

Observation of lipopolysaccharide receptor CD14 involved in acute hepatic failure through nuclear factor-κB pathway in rats

作　　者：黄瑜[1] 陈永平[1] 许烂漫[1] 黄卡特[1] 高峰[1] 王晓东[1] 

机构地区：[1]温州医学院附属第一医院感染内科,325000

出　　处：《中华传染病杂志》2008年第2期74-77,共4页Chinese Journal of Infectious Diseases

基　　金：浙江省引进国外技术、管理人才项目（Y2004GY031）

摘　　要：目的观察CD14、核因子（NF）-κB在D-氨基半乳糖介导的大鼠急性肝功能衰竭模型中的动态变化规律。方法用D-氨基半乳糖诱导大鼠急性肝功能衰竭模型，在造模后6、12、24、36、48、72、120、168、240h用鲎试剂检测门静脉脂多糖（LPS），RT-PCR法检测肝脏CD14mRNA，免疫组织化学检测CD14和NF-κB蛋白表达。实验数据采用两样本t检验及直线相关。结果CD14蛋白和CD14 mRNA在造模后6h升高，48h达高峰（37．13±17．65，0．716±0．089），与健康对照组（2．07±1．84，0．355±0．098）比较，差异有统计学意义（t=-13．236，t=-6．664，P〈0．01），72、120、168和240h逐渐下降。NF-κB蛋白6h升高，48h达高峰（41．97±8．76），与健康对照组（2．33±2．02）比较，差异有统计学意义（t=-24．137，P〈0．01），72h及以后逐渐下降（P〈0．01）。LPS、ALT和AST在6h有轻度升高，至24h与健康对照组比较，差异有统计学意义（P〈0．05），48h达高峰（P〈0．01），72h及以后逐渐下降（P〈0．01）。相关性分析显示，CD14 mRNA、CD14蛋白与ALT、AST、LPS、NF-κB蛋白均有正相关性（r=0．698，P〈0．01）。结论CD14作为LPS的识别受体，可能通过NF-κB途径激活下游细胞因子，引起肝脏损伤。Objective To observe the dynamic change of CD14 and nuclear factor （NF）-κB in acute hepatic failure induced by D-galactosamine in rats. Methods Rat model of acute hepatic failure were established by D-galactosamine. Lipopolysaccharide （LPS） levels in portal vein were detected using tachypleus amebocyte lysate after 6, 12, 24, 36, 48, 72, 120, 168, 240 h after the model was established. The CD14 mRNA was detected by reverse transcriptase polymerase chain reaction （RT- PCR） and the proteins of CD14 and NF-κB were detected by immunohistochemistry. The data was analyzed using two-sample t-test and linear correlation. Results The protein expression and mRNA level of CD14 was significantly increased at 6 h in hepatic failure group than those in healthy control group （P 〈 0.01） and peaked at 48 h （37. 13 ± 17.65 vs 2.07 ± 1.84, 0. 716 ± 0. 089 vs 0. 355 ± 0. 098, t= -13. 236, t= -6. 664, all P 〈 0.01）, then gradually deceased from 72 h （P 〈 0.01）. Levels of LPS, alanine aminotransierase （ALT）, and aspartate aminotransierase （AST） in hepatic failure group were lightly increased at 6 h; significantly different from those in control group at 24 h （P 〈 0. 05） ; peaked at 48 h （P 〈 0.01） and began to decrease at 72 h （P 〈 0. 01）. Correlation analysis demonstrated that CD14 mRNA and the protein of CD14 were positively correlated with ALT, AST, LPS and NF-κB （r=0. 698, P 〈 0. 01）. Conclusion As the recognition receptor of LPS, CD14 maybe activate downstream cytokines through NF-κB pathway and result in liver injure.

关 键 词：肝功能衰竭 D-氨基半乳糖 抗原 CD14 逆转录聚合酶链反应 免疫组织化学 

分 类 号：R575.3[医药卫生—消化系统]