氩氦刀治疗恶性黑色素瘤对小鼠T细胞免疫的影响  

Effect of Cryosurgery on Function of T Cellular Immunity in Mice with Malignant Melanoma Carcinoma

作　　者：骆益宙 钱建新[2] 顾小强[2] 于观贞[2] 王战 武清[2] 王杰军[2] 

机构地区：[1]解放军454医院肿瘤科,南京210002 [2]上海长征医院肿瘤科

出　　处：《华南国防医学杂志》2008年第1期7-8,共2页Military Medical Journal of South China

基　　金：南京军区医药卫生科研项目(06MA71)

摘　　要：目的研究氩氦刀治疗小鼠恶性黑色素瘤后激活特异性T淋巴细胞的免疫效应。方法12只C57/BL小鼠分3组,A组4只接种B16恶性黑色素瘤细胞,并行氩氦刀治疗;B组4只建立荷瘤模型;C组4只为对照。利用混合淋巴细胞反应检测特异性细胞毒性T淋巴细胞的增殖。结果氩氦刀治疗后可显著刺激特异性T淋巴细胞增殖,与对照组比较具有显著性差异(P<0.01)。结论氩氦刀治疗可有效激活小鼠肿瘤抗原特异性的T细胞。Objective To study the effect of cryosurgery on activating T ell immunity in mice with malignant melanoma carcinoma. Methods Twelve C57/BL mice were randomly divided into group A （the mice were transplanted with B16 malignant melanoma cells, then treated by cryosurgery）, group B （only transplanted with B16 cells）and group C as control. The proliferative activities of cytotoxic T lymphocytes were investigated by using mixed lymphocytes reaction. Results After cryosurgery, the cytotoxic T lymphocytes were activated into proliferate effectively. The proliferation rate of cytotoxic T lymphocytes in group A was significantly higher than that in group C （P〈O. 01）. Conclusion Cryosurgery can effectively activate tumor antigen-specific T lymphocytes.

关 键 词：恶性黑色素瘤 氩氦刀 T细胞免疫 

分 类 号：R739.5[医药卫生—肿瘤]