鼠脑钠通道Ⅰ的IS3片段的溶液法合成  

Synthesis of the IS3 Segment of the Rat Brain Sodium Ion Channel

作　　者：苗振伟[1] 蒋云[1] 李向群[1] 徐筱杰[1] 唐有祺[1] 

机构地区：[1]北京大学化学与分子工程学院,北京100871

出　　处：《高等学校化学学报》1997年第5期734-738,共5页Chemical Journal of Chinese Universities

基　　金：国家攀登计划资助

摘　　要：采用本甲酸甲酯作为α－羧基的暂时性保护基，以片段缩合的方式合成了疏水性多肽──鼠脑钢通道Ⅰ的IS3片段，利用高效液相色谱对其进行了纯化，并通过了氨基酸组成分析和快原子轰击质谱鉴定．The IS3 segment, a peptide with 22 amino acid residues in length, of the rat brain sodium ion channel was synthesized by the fragment condensation using the phenacylester as the temporary protection group of a-carboxyl group. After removal of the protection group,the crude product was purified by HPLC first on Protein Parck column and then on C18 column, using 40% acetonitrile in 0. 1 mol/L NH4HCO3 aqueous solution as the mobile phase.The purified IS3 segment was confirmed by amino acid analysis and FAB-MS.

关 键 词：苯甲酰甲酯 多肽 合成 IS3片段 片段缩合 钠通道 

分 类 号：Q514.303[生物学—生物化学]