腾冲两热泉泉古菌多样性及系统发育的初步分析  

Crenarchaeal Diversity and Phylogenetic Analysis of Two Hot Springs in Tengchong

作　　者：宋兆齐[1] 陈经全[1] 职晓阳[1] 黄志勇 张传伦 李文均[1] 

机构地区：[1]云南大学云南省微生物研究所教育部微生物多样性可持续利用重点实验室,昆明650091 [2]Department of Marine Sciences,University of Georgia,Athens,GA 30602,USA

出　　处：《微生物学通报》2008年第3期372-377,共6页Microbiology China

基　　金：国家“973项目”(No.2004CB719601);国家自然科学基金(No.30600001);教育部新世纪优秀人才支持计划(No.NCET-05-0821);云南省中青年学术和技术带头人后备人才基金(No.2005PY01-12)

摘　　要：通过构建16SrRNA基因片段的克隆文库对腾冲热海两温泉中泉古菌的多样性和系统发育关系进行了初步的研究。一共得到18个泉古菌克隆序列,可分为12个OTUs,两温泉的克隆序列与已知GenBank上关系最近序列的平均相似性较低,无名泉为92.56%,热爆区为93%。从基于16SrRNA基因片段序列构建的系统发育树来看,74℃的无名泉样点中既有属于超高温环境类群的泉古菌,同时又有属于和常温环境较接近的泉古菌;45℃的热爆区样点的泉古菌,相对来说则更接近于常温类群。本次研究表明,腾冲热泉与世界其它同类热泉之间的泉古菌类群存在着一定的差异;而且两实验样点代表了超高温和高温环境泉古菌逐渐向常温过度的两个重要环境。The crenarchaeal diversity and phylogenesis of two hot springs in Tengchong were investigated and analyzed using the construction of 16S rRNA gene clone libraries. Total 18 crenarchaeal sequences were obtained and divided into 12 OTUs. The average similarities between the clone sequences from Wuming hot spring and Rebao hot spring and their closest sequences deposited in GenBank is 92.56% and 93%, respectively. Based on the phylogenetic tree constructed by using crenarchaeal 16S rRNA gene sequences, Wuming hot spring not only have hyperthethermophilic crenarchaeal clusters but also have the crenarchaeal clusters that have close phylogenetic relationship with non-thermophilic crenarchaeota. And most of the crenarchaeota in Rebao spring distribute in normal temperature groups. This study showed that crenarchaeal clusters in Tengchong hot springs have some differences with other hot springs in the world, and these springs represent two important environments for investigating the evolutionary origin of the non-thermophilic Crenarchaeoone library.

关 键 词：泉古菌 多样性 系统发育 16S RRNA基因 克隆文库 热泉 腾冲 

分 类 号：Q93[生物学—微生物学]