副溶血性弧菌重复序列-PCR分型研究  

Typing of Vibrio parahaemolyticus by Repetitive Element Sequence-based PCR

作　　者：金莉莉[1] 董雪[2] 王秋雨[1] 李雪[1] 吴琼[1] 李继耀[2] 

机构地区：[1]辽宁大学生命科学学院,沈阳 110036 [2]沈阳市疾病预防控制中心,沈阳 110037

出　　处：《微生物学通报》2008年第3期389-394,共6页Microbiology China

基　　金：沈阳市科技局资助项目(No.1063231-3-00)

摘　　要：利用基因外重复回文序列-PCR(REP-PCR)和肠细菌基因间共有重复序列-PCR(ERIC-PCR)技术,对副溶血性弧菌进行了分子分型研究和亲缘关系的探讨,并使用Hunter和Gaston方法计算分辨力指数。结果显示40株副溶血性弧菌分离株均可扩增产生可重复的DNA指纹图谱,并且不同菌株基因组DNA的扩增条带具有多态性。根据SPSS10.0软件得出的树状图结果,REP-PCR可以把40株菌分为21个型,分辨力指数可达到0.953,优势菌型为G1型;ERIC-PCR可将40株菌分成4个型,分辨力指数为0.5。研究显示重复序列-PCR方法可以用于该菌分型分析,REP-PCR具有较好的分型能力。在两种PCR的DNA指纹图谱中,血清型O1群与O3群主条带均非常相似,表明它们之间亲缘关系密切。Two kinds of Rep-PCR, Repetitive extragenic palindromic elements-PCR（REP-PCR） and en-terobacterial repetitive intergenic consensus sequences-PCR （ERIC-PCR）, were used firstly in typing and genetic relationship research for Vibrio parahaemolyticus in China, and Hunter ＆ Gaston＇s method was used to calculate discriminative index of REP-PCR and ERIC-PCR. Forty Vibrio parahaemolyticus strains, representing a wide range of REP and ERIC patterns, were grouped into 21 and 4 patterns with discriminative index of 0.953 and 0.5, by REP-PCR and ERIC-PCR respectively. G1 type was predominant in 40 strains of Vibrio parahaemolyticus when typed by REP-PCR. The results show that Rep-PCR is feasible for typing Vibrio parahaemolyticus strains, and REP-PCR possesses better typing potential. Similar DNA fingerprints were gained from serotype O1 and 03 strains by two kinds of Rep-PCR. This result indicate that the genetype of serovar 03 and O1 are closely related.

关 键 词：副溶血性弧菌 基因外重复回文序列-PCR肠细菌基因问共有重复序列-PCR 分型 亲缘关系 

分 类 号：R378[医药卫生—病原生物学]