促进玉米秸秆酶解效率的化学预处理方法比较  

Comparative Study on Chemical Pretreatments to Accelerate Enzymatic Hydrolysis of Corn Straw

作　　者：柯静[1] 余洪波[1] 徐春燕[1] 张晓昱[1] 

机构地区：[1]华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《纤维素科学与技术》2008年第1期7-12,共6页Journal of Cellulose Science and Technology

摘　　要：分别用八种化学方法对玉米秸秆进行预处理,将预处理后的试样用纤维素酶在最优条件下催化水解,初步比较了不同的化学方法在促进玉米秸秆酶解糖化方面的效果。通过比较各试样酶解后产糖量大小,得到最佳的预处理方案:采用0.176%(m/V)NaOH及0.9%(V/V)H2O2混合液在常温下按固液比1∶50振荡作用24 h,即在纤维素酶用量为50 FPU/g时,产糖量可从0.055 g/g提升到0.333 g/g,提高了83.51%;此时的木质素降解量亦为最大,达到了49.8%,此结果表明木质素的降解有利于纤维素酶敏感性的提高。Eight kinds of chemical pretreatment on com straw to accelerate enzymatic hydrolysis were comparatively examined. The factor for evaluation is the yields of reduced sugar by enzymatic hydrolysis of each specimen of corn straw. As a result, the alkali/oxidative [0.1.76% （m/V） NaOH and 0.9% （V/V） H2O2] pretreatment for 24 h was the most effective method. When the cellulase that used for 1 g corn straw was 50 FPU, the net yield of reduced sugar is 0.333 g/g （corn straw）. Compared to the one untreated, this made the net yields of reduced sugar increased by 83.5%. At the same time, the lignin in this specimen reduced by 49.8%, which induced that the removing of lignin in corn straw could enhance susceptivity of cellulase.

关 键 词：玉米秸秆 化学预处理 酶解 还原糖 木质素 

分 类 号：Q93[生物学—微生物学] TS721.1[轻工技术与工程—制浆造纸工程]