乳中环核苷酸提取方法研究  

Extraction Method of Cyclic Nucleotieds of Milk

作　　者：马志科[1] 昝林森[1] 王倩[1] 张恩平[1] 孙超[1] 

机构地区：[1]西北农业大学动物科学系

出　　处：《西北农业大学学报》1997年第5期29-38,共10页Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)

基　　金：陕西省"八五"攻关资助;西北农业大学青年基金

摘　　要：根据环核苷酸理化性质及乳的特点，采用酶法从乳汁中提取环核苷酸。研究表明，该方法条件温和、工艺合理、操作方便。经放射免疫测定提取浓缩液浓度为ｃＡＭＰ２０μｍｏｌ·Ｌ－１，ｃＧＭＰ０．２μｍｏｌ·Ｌ－１．Based on physical and chemical properties of cyclic nucleotydes and the speciality of milk,cyclic nucleotides in milk were extracted with enzyme method.The study showed that the method was moderate in condition, reasonable in technique and easy in operation.The extracted fluid concentration of cAMP and cGMP were,by radioimmunoassay detction, 20 μmol·L -1 and 0.2 μmol·L -1 respectively.

关 键 词：乳液 环核苷酸 提取方法 酶法 

分 类 号：Q524.03[生物学—生物化学]