环孢菌素A、汉防己甲素及两者联合逆转K562/A02细胞的多药耐药  

In Vitro study of the effect of cyclosporine A,tetrandrine and their combination on the reversion of multidrug resistance in leukemia cell line

作　　者：郭晶晶[1] 陈宝安[1] 高峰[1] 丁家华[1] 许文林[2] 沈惠玲[2] 孙新臣[3] 成红艳[3] 高冲[1] 孙耘玉[1] 程坚[1] 王骏[1] 赵刚[1] 宋慧慧[1] 

机构地区：[1]东南大学附属中大医院血液科,江苏南京210009 [2]江苏大学附属人民医院血液科,江苏镇江212002 [3]东南大学附属中大医院肿瘤科,江苏南京210009

出　　处：《东南大学学报（医学版）》2008年第2期82-86,共5页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目(39970832;30740062);江苏省科技发展计划(社会发展)项目(BS20020224)

摘　　要：目的:探讨环孢菌素A(CsA)、汉防己甲素(Tet)及两者联合应用对人白血病耐药细胞系K562/A02多药耐药的逆转作用。方法:采用MTT法测定柔红霉素(DNR)对细胞的半数抑制量(IC50),流式细胞仪检测细胞凋亡率和细胞内DNR浓度。结果:DNR对K562细胞和K562/A02细胞IC50分别为0.27和21.22 mg.L-1;CsA(1 mg.L-1)及Tet(0.1 mg.L-1)单独和联合处理K562/A02后,DNR的IC50分别为7.83、5.32和2.32 mg.L-1;CsA和Tet对DNR作用K562的IC50无明显影响;K562/A02细胞48 h凋亡率为2.19%,DNR(1 mg.L-1)作用K562/A02细胞凋亡率为2.70%,CsA和Tet单独及联合处理后凋亡率分别为10.27%、17.64%和52.79%;两药处理后细胞内化疗药物DNR浓度亦明显增加。结论:CsA及Tet均可逆转耐药,且联合作用逆转效果更强。To study the reversible effect of cyclosporine A（ CsA）, tetrandrine（Tet） and their combination on multidrug resistance of cell line K562/A02. Methods The IC50 of DNR was determined by MTT assay. The apoptotic changes and the intracellular DNR concentration were observed by flow cytometry. Results Treating K562/A02 cells with CsA（ 1 mg·L^-1 ） ,Tet（0.1 mg·L^-1） and both for 48 hours partially restored the sensitivity of K562/A02 cells to DNR, but had no effect on K562 cells;K562/A02 cells showed apoptotic characteristics after treated with CsA and both for 48 hours; CsA and Tet （alone or combination） elevated the intracellular DNR concentration in K562/A02 cells. Conclusion Multidrug resistance can be partially reversed by CsA or Tet, the combination of both drugs shows a great reversal effect.

关 键 词：环孢菌素A 汉防己甲素 K562/A02细胞 多药耐药 

分 类 号：R733.7[医药卫生—肿瘤] R969[医药卫生—临床医学]