不同时程吗啡依赖戒断大鼠海马CA1区BDNF及PSD-95的表达  

The Expression of BDNF and PSD-95 in Hippocampal CA1 Region of Morphine-withdrawn Rat with Different Dependent Times

作　　者：韩军鸽[1] 朱华[1] 陈国弟[2] 陈鹏[3] 罗良鸣[3] 刘晓妮[1] 王程宝[1] 

机构地区：[1]温州医学院法医学系,温州325035 [2]四川大学华西基础医学与法医学院法医物证学教研室 [3]温州市公安局

出　　处：《四川大学学报（医学版）》2008年第2期253-255,共3页Journal of Sichuan University(Medical Sciences)

基　　金：浙江省教育厅(20051166);温州市科技局(Y2005A039)资助

摘　　要：目的观察不同时程吗啡依赖大鼠戒断1周后海马CA1区脑源性神经生长因子(BDNF)及突触后致密质95(PSD-95)的表达变化,探讨吗啡依赖戒断对海马CA1区的影响。方法建立不同时程(1周、2周、4周)吗啡依赖大鼠模型并自然戒断1周后,应用RT-PCR方法观察海马CA1区BDNF及PSD-95的表达情况。结果吗啡依赖1周戒断组大鼠海马CA1区的BDNF及PSD-95表达较生理盐水对照组下降(P<0.01),吗啡依赖2周戒断组大鼠海马CA1区的BDNF及PSD-95表达较吗啡依赖1周戒断组大鼠有所上升(P<0.05),但仍低于生理盐水对照组(P<0.01),吗啡依赖4周戒断组大鼠海马CA1区的BDNF及PSD-95表达较吗啡依赖1周戒断组大鼠、吗啡依赖2周戒断组大鼠均下降(P<0.01)。结论BDNF及PSD-95在吗啡依赖大鼠自然戒断1周后的海马CA1区的表达降低,吗啡依赖戒断对海马CA1区损伤明显。Objective To observe the expression of brain-derived neurotrophic factor （BDNF） and postsynaptic density-95（PSD-95） in hippocampal CA1 region of rat with morphine dependence for different times and withdrawn for 1 week, and investigate the influence of that morphine dependence is withdrawn on rat hippocampal CA1 area. Methods Animal models of rats with morphine withdrawal for 1 week and different morphine dependent times（1 week,2 weeks,4 weeks） were established. The expression of BDNF and PSD-95 in hippocampal CA1 were identified with RT-PCR. Results The expression of BDNF and PSD-95 in hippocampal CA1 decreased in the withdrawn group with morphine dependence for 1 week as compared with that in normal saline （NS） group（P〈0. 01）, and it increased in the withdrawn group with morphine dependence for 2 weeks as compared with that in morphine-dependent group for 1 week （P〈0. 05） but still decreased as compared with that in NS group （P〈0. 01）, and it decreased in the withdrawn group with morphine dependence for 4 weeks as compared with the other three groups （P〈0. 01）. Conclusion The expression of BDNF and PSD-95 in hippocampal CA1 decreases in morphine-depended rats withdrawn for 1 week. Morphine withdrawal has a manifest harm in hippocampal CA1 region of rat.

关 键 词：吗啡戒断 海马 BDNF PSD-95 

分 类 号：R749.64[医药卫生—神经病学与精神病学]