扁绒泡菌Physarum compressum原质团细胞核核骨架制备技术的研究  

Nuclear matrix of Physarum compressum phanero-plasmodium

作　　者：王晓丽[1] 李玉[1] 

机构地区：[1]吉林农业大学茵物研究所,长春130118

出　　处：《菌物学报》2008年第2期177-182,共6页Mycosystema

基　　金：国家自然科学基金项目(No.30070624);吉林农业大学博士启动基金(No.203136)

摘　　要：以扁绒泡菌显型原质团为材料,进行细胞核的提纯、核骨架的制备及电镜观察。结果表明:用2mol/LNaCl+TritonX-100/NP40可得到具有复合纤维的核骨架,而用Lis+TritonX-100/NP40得到的核骨架具有核纤层,不使用RNase得到了具有网状的细胞核骨架,RNA在核骨架的结构形态中起重要作用。阐述了原质团中游离细胞核在细胞生物学研究中的意义。Nuclear matrix prepared from phaneroplasmodium of Physarum compressum was observed under TEM. The nuclear matrix with compound-fibres was obtained by use of 2mol/L NaCI ＋ TritonX-100/NP40, while nuclear matrix with nuclear lamina was obtained by using Lis ＋ TritonX-100/NP40. Net nuclear matrix was obtained without using RNase, showing RNA played an important role in nuclear matrix structure. The significance of dissociated nuclei of plasmodium in the study of cell biology was expounded in this paper.

关 键 词：黏菌 营养生长阶段 细胞器 核基质 

分 类 号：Q93[生物学—微生物学]