亚稀褶黑菇和稀褶黑菇的ITS序列分析  

Sequence analysis of the internal transcribed spacer of gene coding for rDNA in Russula subnigricans and R. nigricans

作　　者：尹军华[1] 张平[1] 龚庆芳[1] 陈作红[1] 

机构地区：[1]湖南师范大学生命科学学院,长沙410081

出　　处：《菌物学报》2008年第2期237-242,共6页Mycosystema

基　　金：国家自然科学基金(No.30471208)

摘　　要：对亚稀褶黑菇和稀褶黑菇的ITS全序列进行了测定和比较,首次报道了亚稀褶黑菇的ITS1和5.8S区域。在ITS区域中,不同采集地的亚稀褶黑菇与稀褶黑菇的5.8SrDNA具有100%的同源性,而两侧ITS之间表现出种内和种间的多态性,种内的差异均不超过5%,种间的差异达10%左右。ITS序列分析方法可以作为两者的鉴定方法。关键词:rDNA,多态性,红菇属。The internal transcribed spacer of gene coding for rDNA of Russula subnigricans and Russula nigricans were sequenced and analyzed, and 5.8S rDNA sequences and ITS1 sequences of R. subnigricans were reported for the first time. The results show that there is no difference between 5.8S rDNA sequences of R. subnigricans and R. nigricans, but the length discrepancy and sequence polymorphism of ITS1 and ITS2 are existed between the two species. Their intraspecies distinctions did not exceed 5%, but their interspecies distinctions were about 10%. The ITS sequence can be used as identification method for R. subnigricans and R. nigricans.

关 键 词：RDNA 多态性 红菇属 鉴定方法 

分 类 号：S646[农业科学—蔬菜学]