红托竹荪菌托多糖的提取及抗肿瘤活性的初步研究  

Extraction and primary investigation of antitumor activity of polysaccharides from the volva of Dictyophora rubrovolvata

作　　者：赵凯[1] 王飞娟[1] 潘薛波[1] 朱诚[1] 

机构地区：[1]浙江大学生命科学学院植物生理学与生物化学国家重点实验室,杭州310058

出　　处：《菌物学报》2008年第2期289-296,共8页Mycosystema

基　　金：浙江省科技计划项目(No.2006C23015)

摘　　要：红托竹荪菌托经热水提取、酒精沉淀、脱蛋白后的粗多糖,其得率远大于其菌丝体和子实体的其他部位及香菇子实体。菌托粗多糖进一步用DEAE纤维素柱和Sephadex G-75分离纯化,得到两个组分DRVP1与DRVP2,对分离得到的主要多糖通过高效液相色谱(HPLC)、红外光谱(IR)等进行结构分析。DRVP1的相对分子质量(Mr)为1.47×104,红外光谱数据显示为β-型甘露糖苷。体外试验表明,红托竹荪菌托多糖的组分DRVP1对小鼠S180肉瘤有一定的抑制作用。The polysaccharides from the volva of Dictyophora rubrovolvata were extracted by hot water, precipitated by alcohol in different concentrations and deproteinized by the method of Sevag. The yield of crude polysaccharides of the volva were much higher than that of mycelium and other parts of fruit body. Two kinds of polysaccharides, DRVP1 and DRVP2, were purified by use of column chromatography on DEAE-cellulose and Sephadex G-75, and the structural features of the polysaccharides were elucidated by HPLC and IR respectively.The inhibitory effect of DRVP1 on the tumor cells of S180 （Sorcoma-180） was investigated by sulforhodamine-B （SRB） methods. The result showed that the molecular weight of DRVP1 was 1.47×10^4, and it had an inhibitory effect on the tumor cells of S180 to some extent.

关 键 词：食用菌 分离纯化 分子量 结构分析 红外光谱 

分 类 号：S646.8[农业科学—蔬菜学]