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作 者:李碧侠[1] 任守文[1] 王学敏[1] 葛云山[1]
机构地区:[1]江苏省农科院畜牧研究所,江苏南京210014
出 处:《家畜生态学报》2008年第1期17-20,24,共5页Journal of Domestic Animal Ecology
基 金:江苏省科技厅高技术资助(BG2005305);江苏省农科院基金资助(6110215)
摘 要:通过PCR-RFLP方法,检测外来品种大约克猪和江苏地方品种梅山猪、二花脸猪、姜曲海猪以及培育品种苏钟猪共144头,结果表明PGC1基因第8外显子序列经Aul I酶切后存在AA、AT和TT 3种基因型,其中大约克猪中AA基因型占优势,A等位基因频率为0.7,而江苏地方品种猪中,TT占绝对优势。分析PGC1基因型与胴体性状相关性发现,AA基因型猪的左胴重、右胴重和脂肪重均高于TT基因型猪,差异达到显著水平(P<0.05)。倒数第三、四腰椎间背膘厚AA基因型显著高于TT基因型猪,差异达到极显著水平(P<0.01),AT基因型与TT基因型差异达到显著水平(P<0.05)。The genetic variations of the eighth exon of PGC1 gene were detected with PCR-RFLP-Alu Ⅰ in 144 pigs including Meishan pig, Erhualian pig,Jiangquhai pig, Suzhong pig and Yorkshire. The results showed that there were three genotypes (AA, AT, TT) and the genotype of AA was dominant in Yorkshire, and the frequency of allele A was 0. 7. In the Jiangsu native breeds, the genotype of TT was dominant. The relation of PGC1 gene and carcass traits indicated that the value of left carcass, right carcass and fat weight for AA genotype pig were higher than those of TT genotype pig( P 〈 0. 05 ). Backfat at vertebra lumbar of AA genotype pig was significantly higher than those of TT genotype pig( P〈 0. 05 ), AT genotype pig higher than TT genotype pig( P〈0. 05).
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