Isolation and bioinformatics analysis of differentially methylated genomic fragments in human gastric cancer  

作　　者：Ai-Jun Liao Qi Su Xun Wang Bin Zeng Wei Shi 

机构地区：[1]Cancer Research Institute, Nanhua University, The First Affiliated Hospital, University of South China, Hengyang 421001, Hunan Province, China [2]Department of Gastroenterology, The First Affiliated Hospital, University of South China, Hengyang 421001, Hunan Province, China

出　　处：《World Journal of Gastroenterology》2008年第9期1333-1338,共6页世界胃肠病学杂志（英文版）

基　　金：The Key Project Fund of the Science and Technology Program of Hunan Province, No. 04SK1004;the Scientific Research Fund of Educational Department, Hunan Province, No. 99C195

摘　　要：AIM:To isolate and analyze the DNA sequences which are methylated differentially between gastric cancer and normal gastric mucosa. METHODS: The differentially methylated DNA sequences between gastric cancer and normal gastric mucosa were isolated by methylation-sensitive representational difference analysis (MS-RDA). Similarities between the separated fragments and the human genomic DNA were analyzed with Basic Local Alignment Search Tool (BLAST). RESULTS: Three differentially methylated DNA sequences were obtained, two of which have been accepted by GenBank. The accession numbers are AY887106 and AY887107. AY887107 was highly similar to the 11th exon of LOC440683 (98%), 3' end of LOC440887 (99%), and promoter and exon regions of DRD5 (94%). AY887106 was consistent (98%) with a CpG island in ribosomal RNA isolated from colorectal cancer by Minoru Toyota in 1999. CONCLUSION: The methylation degree is different between gastric cancer and normal gastric mucosa. The differentially methylated DNA sequences can be isolated effectively by MS-RDA.AIM：To isolate and analyze the DNA sequences which are methylated differentially between gastric cancer and normal gastric mucosa. METHODS： The differentially methylated DNA sequences between gastric cancer and normal gastric mucosa were isolated by methylation-sensitive representational difference analysis （MS-RDA）. Similarities between the separated fragments and the human genomic DNA were analyzed with Basic Local Alignment Search Tool （BLAST）. RESULTS： Three differentially methylated DNA sequences were obtained, two of which have been accepted by GenBank. The accession numbers are AY887106 and AY887107. AY887107 was highly similar to the 11th exon of LOC440683 （98%）, 3＇ end of LOC440887 （99%）, and promoter and exon regions of DRD5 （94%）. AY887106 was consistent （98%） with a CpG island in ribosomal RNA isolated from colorectal cancer by Minoru Toyota in 1999. CONCLUSION： The methylation degree is different between gastric cancer and normal gastric mucosa. The differentially methylated DNA sequences can be isolated effectively by MS-RDA.

关 键 词：Gastric cancer DNA methylation Differentia sequences Methylation-sensitive representationa difference analysis 

分 类 号：R735.2[医药卫生—肿瘤]