Activator protein-1 involved in growth inhibition by RASSF1A gene in the human gastric carcinoma cell line SGC7901  

作　　者：Zheng-Hao Deng Ji-Fang Wen Jing-He Li De-Sheng Xiao Jian-Hua Zhou 

机构地区：[1]Department of Pathology, Xiangya School of Medicine, Central South University, Changsha 410078, Hunan Province, China

出　　处：《World Journal of Gastroenterology》2008年第9期1437-1443,共7页世界胃肠病学杂志（英文版）

摘　　要：AIM:To investigate the role of Ras association domain family protein 1 isoform A (RASSF1A) in gastric tumorigenesis. METHODS:Through over-expression of RASSF1A gene in the SGC7901 cell line which was induced by a lipofectamine-mediated gene transfer approach. Activator protein-1 (AP-1) DNA binding activity was measured by electrophoretic mobility shift assay (EMSA). RESULTS:Compared with the control clones, cells over- expressing RASSF1A exhibited significant inhibition of cell growth with G1 cell cycle arrest in vitro and in vivo. The over-expression of RASSF1A significantly inhibited AP-1 activity in SGC7901 cells (0.981±0.011 vs 0.354±0.053, P<0.001). In addition, both Western blot analysis and immunocytochemistry demonstrated that RASSF1A down-regulated the expression of c-Fos (0.975± 0.02 vs 0.095±0.024, P<0.001) but not c-Jun. CONCLUSION: Over-expression of RASSF1A inhibits the growth of SGC7901 cells by negatively regulating the AP-1 activity, the latter in turn negatively signals cell proliferation.AIM：To investigate the role of Ras association domain family protein 1 isoform A （RASSF1A） in gastric tumorigenesis. METHODS：Through over-expression of RASSF1A gene in the SGC7901 cell line which was induced by a lipofectamine-mediated gene transfer approach. Activator protein-1 （AP-1） DNA binding activity was measured by electrophoretic mobility shift assay （EMSA）. RESULTS：Compared with the control clones, cells over- expressing RASSF1A exhibited significant inhibition of cell growth with G1 cell cycle arrest in vitro and in vivo. The over-expression of RASSF1A significantly inhibited AP-1 activity in SGC7901 cells （0.981±0.011 vs 0.354±0.053, P〈0.001）. In addition, both Western blot analysis and immunocytochemistry demonstrated that RASSF1A down-regulated the expression of c-Fos （0.975± 0.02 vs 0.095±0.024, P〈0.001） but not c-Jun. CONCLUSION： Over-expression of RASSF1A inhibits the growth of SGC7901 cells by negatively regulating the AP-1 activity, the latter in turn negatively signals cell proliferation.

关 键 词：RASSFIA Gastric adenocarcinoma SGC7901  Activator protein-1 

分 类 号：R735.2[医药卫生—肿瘤]