荧光定量聚合酶链反应检测儿童白血病骨髓GRIK2基因的表达  

Expression of Gene GRIK2 in Leukemia Children's Bone Marrow by Fluorescent Quantitation-Polymerase Chain Reaction Method

作　　者：胡婷[1] 王卓[1] 曹励之[1] 俞燕[1] 康睿[1] 许望琼[1] 

机构地区：[1]中南大学湘雅医院儿科,长沙410008

出　　处：《实用儿科临床杂志》2008年第3期179-181,236,共4页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金项目资助(30571982;30772353)

摘　　要：目的分析骨髓中GRIK2基因产物在白血病患儿、白血病细胞株及非血液病患儿中的表达差异。方法采用反转录聚合酶链反应(RT-PCR)和荧光定量PCR(FQ-PCR)方法分别检测100例白血病患儿、3个白血病细胞株(Jurkat、Raji和HL-60细胞株)及30例对照组骨髓中GRIK2的表达情况。结果RT-PCR和FQ-PCR检测标本的阳性率分别为4.51%(6/133例)和7.52%(7/133例),2种检测方法均显示GRIK2基因在白血病和正常造血细胞间的表达无统计学差异(P>0.05),但GRIK2mRNA在无6q缺失的T-急性淋巴细胞白血病(ALL)患儿中较B-ALL患儿表达高,在有6q缺失的T-ALL和B-ALL患儿中GRIK2表达为阴性,T细胞株Jurkat有阳性表达,B细胞株Raji和HL-60细胞株为阴性表达;30例对照组中3例有较高水平表达。结论GRIK2mRNA在骨髓中水平低或不表达,GRIK2基因是否为ALL的肿瘤抑制基因(TSG)有待进一步探讨。Objective To observe GRIK2 gene＇s expression circumstance among leukemia cell lines, patients＇ and control groups＇ bone marrow, and analyze the expression difference of the GRIK2 gene outcomes. Methods Methods of reverse transcriptase - polymerase chain reaction （RT- PCR） and fluorescent quantitation（FQ） -PCR were used to examine the expression of GRIK2 gene in 3 leukemia cell lines （Jurkat cell lines, Raji cell lines and HL -60 cell lines） of 100 patients＇ and 30 control groups＇ bone marrow. Results The positive ratio respectively was 4.51% （6/133 cases） and 7.52% （7/133 cases） by using RT - PCR and FQ - PCR to examine all specimens. The difference of GRIK2 expression between leukemia cells and normal cells had no statistics meaning （P 〉 0.05 ）. However, the GRIK2 mRNA expresses higher in T - acute lymphoblastic leukemia （ALL） of no 6q deficiency than B - ALL;while which had the 6q deficiency were negative. Jurkat expresses positive, Raji and HL -60 expresses negative. Three had higher level of expression in controls. Conclusions The expression levels of GRIK2 mRNA were low in bone marrow. GRIK2 gene whether or not is tumor -suppressing gene（TSG） of ALL needs the further study.

关 键 词：白血病 淋巴细胞 急性 肿瘤抑制基因 GRIK2 荧光定量聚合酶链反应 儿童 

分 类 号：R733.7[医药卫生—肿瘤]