谷胱甘肽S-转移酶M1/T1基因多态性与儿童急性淋巴细胞白血病的相关性  

Relationship between Glutathione S-Transferase M1/T1 Genetic Polymorphisms and Childhood Acute Lymphocytic Leukemia

作　　者：张利[1] 王军[1] 冯建飞[1] 王宏[1] 朱绍先[1] 张欣欣[1] 

机构地区：[1]郑州大学第三附属医院儿童保健科,郑州450052

出　　处：《实用儿科临床杂志》2008年第3期195-197,共3页Journal of Applied Clinical Pediatrics

基　　金：河南省科委科技攻关项目资助(001170121)

摘　　要：目的研究谷胱甘肽S-转移酶(GST)M1/T1基因多态性与儿童急性淋巴细胞性白血病(ALL)易感性和预后的相关性。方法研究组为67例ALL患儿,对照组为146例健康献血员。分别留取外周血标本,提取基因组DNA,用PCR方法测定其GSTM1/T1基因型;同时记录患者的发病年龄、性别、发病时外周血白细胞数、FAB形态学分型,早期治疗反应等影响患者预后的临床资料,并分析GSTM1/T1基因型与这些高危因素之间的关系。结果ALL患儿GSTM1、GSTM1/T1纯合子缺失率显著高于对照组(P=0.001,P=0.0001);ALL患儿GSTT1基因纯合子缺失率与对照组比较无显著性差异(P>0.05)。GSTM1/T1基因型与患儿的发病年龄、性别、发病时外周血WBC、FAB形态学分型等均无相关性(Pa>0.05)。GSTM1基因纯合子缺失者的早期治疗反应与有GSTM1功能性等位基因的患儿比较无显著性差异(P=0.733)。但GSTT1基因纯合子缺失的患者发生早期治疗反应差的风险较小(P=0.080)。结论GSTM1纯合子缺失基因型是儿童发生ALL的危险基因型,而GSTT1基因纯合子缺失者早期治疗效果较好。Objective To study the relationship between glutathione S - transferase（GST） M1/T1 genetic polymorphisms and the etiology and outcome of childhood acute lymphocytic leukemia（ALL）. Methods The cases were the 67 ALL children and the controls were the 146 healthy blood donors. Their genomic DNA were extracted from the peripheral blood samples. Using the polymerase chain reaction （PCR） , the GSTM1/T1 genotype were determined. Some clinic data which might influence the outcome of ALL children should be collected. Sex,age and peripheral white blood cell （WBC） at diagnosis. FAB subgroups and initial therapy reaction. And the associations between GSTM1/T1 genotype and some high - risk factors for the outcome of childhood ALL were analyzed. Results The frequencies of GSTM1 and GSTM1/T1 null genotypes significantly increased in ALL cases compared with controls （ P = 0. 001 ,P = 0. 0001 ） ; but the frequencies of GSTI＇I null genotype in ALL cases were not statistically different from the controls （P 〉 0.05）. Examination of sex, age at diagnosis, WBC count at diagnosis, FAB subgroups of children with ALL showed no statistically significant association with GSTM1 or GSTT1 null genotype （Pa 〉 0.05 ）. GSTT1 null genotype conferred a reduction in risk of initial therapy poor - response compared to indiriduals who were either heterozygous or homozygous for GSTrl （P = 0. 080） whereas GSTM1 null genotype showed no significant association with initial therapy response （P = 0. 733 ）. Conclusion The GSTM1 null genotype can be a significant risk factor for children with ALL, but the ALL patients with GSTI＇I null genotype will show a better initial therapy response.

关 键 词：谷胱甘肽S-转移酶 多态性 白血病 淋巴细胞性 急性 

分 类 号：R733.71[医药卫生—肿瘤]