超氧自由基对谷氨酸摄取的抑制及Ebselen的保护  

Inhibition of Glutamate Uptake by Superoxide Anion Radical in Rat Cortical Synaptosomes and the Protective Effect of Ebselen

作　　者：易永[1] 杨祥良[1] 徐辉碧[1] 赵西龙[2] 张亨山[2] 秦钰慧[2] 

机构地区：[1]华中理工大学化学系 [2]中国预防医学科学院环境卫生监测所毒理室

出　　处：《生物化学与生物物理学报》1997年第5期510-512,共3页

摘　　要：超氧自由基对谷氨酸摄取的抑制及Ｅｂｓｅｌｅｎ的保护易永杨祥良徐辉碧＊（华中理工大学化学系，武汉４３００７４）赵西龙张亨山秦钰慧（中国预防医学科学院环境卫生监测所毒理室，北京１０００２１）关键词大脑皮层突触体；谷氨酸摄取；Ｎａ＋，Ｋ＋－ＡＴＰａｓｅ；超...The inhibition of glutamate uptake by superoxide anion radical (O - 2 · ) in rat cortical synaptosomes and the protective effect of Ebselen was studied by radioisotope method. The exposure to xanthine/xanthine oxidase, a O - 2 · generating system, resulted in a marked decrease of high affinity glutamate transport in the synaptosomes. In parallel, the Na +, K + ATPase activity was also damaged, while the lactate dehydrogenase activity and the TBARS contents in synaptic culture were not affected. It suggests that the inhibition of glutamate uptake is related to the damage of Na +, K + ATPase by O - 2 · . Ebselen was showed to have a blocking effect on the glutamate uptake inhibition by O - 2 · ,probably through protecting the Na +, K + ATPase.

关 键 词：谷氨酸 摄取 EBSELEN 超氧自由基 神经系疾病 

分 类 号：Q517.02[生物学—生物化学] R741.02[医药卫生—神经病学与精神病学]