肠衣中醋酸甲羟孕酮残留ELISA检测方法的应用  

Application of ELISA for Detectiong the Residue of MPA in Casing

作　　者：刘烜[1] 贺艳[1] 郑文杰[1] 王莲 陈冰君 王雄 

机构地区：[1]天津出入境检验检疫局,天津100456 [2]北京中检维康技术有限公司,北京100044

出　　处：《中国国境卫生检疫杂志》2008年第1期43-44,50,共3页Chinese Journal of Frontier Health and Quarantine

基　　金：国家质量监督检验检疫总局技术标准专项课题:(2006B)

摘　　要：〔目的〕为醋酸甲羟孕酮残留检测提供一种快速、灵敏的分析方法。〔方法〕利用MPA-BSA免疫新西兰大耳白兔获得抗醋酸甲羟孕酮的多克隆抗体。建立了MPA的ciELSIA方法,并对肠衣样品进行了样品添加回收试验。〔结果〕MPA浓度在0.4～50ng/ml范围内具有良好的线性关系,线性方程为y=6.29-1.7!,r2=0.9995,IC50为3.7ng/ml,向样品中分别添加1、2、5ng/g3个浓度水平的MPA标准品,回收率为85%～110%之间,变异系数为7.1%～9.9%,最低检测限0.8ng/g。药物交叉反应性试验符合要求。〔结论〕该ELSIA方法快速、灵敏、方便,满足了醋酸甲羟孕酮残留检测的要求。Objective To explor a rapid, sensitive assay for detecting the residuce of MPA in casing. Method The MPA-BSA was used to immunize New Zealand white rabbits and the specific antiserum against the antigen was obtained. ELISA of MPA has been established. The standard ELISA inhibition curve for the detection of MPA was established. Result The lineasr ranged 0.4-50 ng/ml, and the regression equation was y = 6.29-1.7χ, r^2= 0,9995,and IC50was 3.7 ng/ml. When casing samples were spiked with 1、2and 5 ng/g respectively, the recovery rate was 85%-110%. The average inter-assay and intra-assay coefficients of variation in the measurement samples of MPA was 7.1%-9.9% and 10.5%-12.5%. The deteetion limit was 0.8 ng/g. Conclusion This ELISA method could determine MPA quickly and economically as possible and could meet the requirement for detecting MPA residence in casing.

关 键 词：醋酸甲羟孕酮 残留 酶联免疫吸附试验 肠衣 

分 类 号：R155.5[医药卫生—营养与食品卫生学]