Utilization of winery wastes for Trichoderma viride biocontrol agent production by solid state fermentation  

作　　者：BAI Zhihui JIN Bo LI Yuejie CHEN Jian LI Zuming 

机构地区：[1]School of Earth and Environmental Science, University of Adelaide, Adelaide, SA 5005, Australia [2]Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China [3]Wastewater Research Unit, South Australian Water Cooperation, Bolivar, SA 5110, Australia [4]Environmental Protection Bureau of Shifiazhuang City, Shijiazhuang 050021, China [5]Teacher＇s College of Beijing Union University, Beijing 100011, China

出　　处：《Journal of Environmental Sciences》2008年第3期353-358,共6页环境科学学报（英文版）

基　　金：supported by the Australian Research Council International Linkage Fellowship(No.LX0560210);the National Natural Science Foundation of China(No.30600082).

摘　　要：Biocontrol agents are safe and environmental friendly alternatives for pesticides in agriculture application. Trichoderma viride WEBL0703 performed a high level of antagonistic activity toward a broad spectrum of phytopathogens and was determined as a biocontrol agent, which was produced by solid state fermentation using grape marc and wine lees. The maximum yield of T. viride conidia was up to 6.65 × 10^9 CFU/g initial dry substrate （IDS） after 10 d fermentation. As important enzymes for protecting plants from disease, chitinase, β-glucanase, and pectinase yields were 47.8 U/g IDS, 8.32 U/g IDS and 9.83 U/g IDS, respectively. These results show that it is feasible to convert winery wastes to a value-added and environmental friendly biocontrol agent.Biocontrol agents are safe and environmental friendly alternatives for pesticides in agriculture application. Trichoderma viride WEBL0703 performed a high level of antagonistic activity toward a broad spectrum of phytopathogens and was determined as a biocontrol agent, which was produced by solid state fermentation using grape marc and wine lees. The maximum yield of T. viride conidia was up to 6.65 × 10^9 CFU/g initial dry substrate （IDS） after 10 d fermentation. As important enzymes for protecting plants from disease, chitinase, β-glucanase, and pectinase yields were 47.8 U/g IDS, 8.32 U/g IDS and 9.83 U/g IDS, respectively. These results show that it is feasible to convert winery wastes to a value-added and environmental friendly biocontrol agent.

关 键 词：grape marc wine lees biocontrol agent Trichoderrna viride solid state fermentation 

分 类 号：X17[环境科学与工程—环境科学]