食管癌核基质抗体的免疫组化研究  

Immunohistochemical study of nuclear matrix antibody in human esophageal cancer

作　　者：李启明[1,2] 刘勇[1,2] 熊华淇[1,2] 吴绮明[1,2] 邹桂华 

机构地区：[1]江西省人民医院病理科 [2]江西医学院病理解剖学教研室

出　　处：《新消化病学杂志》1997年第9期562-563,共2页

基　　金：江西省科委科研基金

摘　　要：目的研究食管癌核基质抗体的肿瘤特异性和组织学特异性．方法用人食管癌组织提取核基质抗原，制备核基质抗体，采用免疫组织化学方法对食管癌４１例（男２６例，女１５例，年龄５３岁±７２岁）、正常食管粘膜８例、食管粘膜异型增生１５例、肺鳞癌１０例、喉癌１０例、胃腺癌１０例以及大鼠食管癌５例进行免疫组化染色．结果食管癌核基质抗体在食管癌组织中表达有特异性（３２／４１，７８０％），与正常食管粘膜（１／８，１２５％）及胃腺癌（２／１０，２００％）有非常显著差异（Ｐ＜００１），与食管异常增生（７／１５，４６７％）、肺鳞癌（３／１０，３００％）、喉鳞癌（４／１０，４００％）差异明显（Ｐ＜００５），但与大鼠食管癌组织（２／５，４００％）差异不明显．食管癌核基质的表达，在不同分化程度的食管癌组织中无明显差异（Ｐ＞００５）；在淋巴结转移阳性组高于淋巴结转移阴性组（１７／１８，９４４％ｖｓ１５／２３，６５２％，Ｐ＜００５）．结论食管癌核基质抗体具有较好肿瘤和组织学特异性，对肿瘤转移有一定影响，可作为食管癌的一项新标记物．AIM To study esophageal cancer nuclear matrix (ECNM) in human esophageal cancer. METHODS By immunohistochemical method, human ECNM expression in tissues of 41 cases of human esophageal caner, 10 cases of lung cancer, 10 of gastric cancer and 5 of mouse esophageal cancer were studied. RESULTS The differences between esophageal cancer (32/41, 78 0%) and lung cancer (3/10, 30 0%), gastric cancer (2/10, 20 0%) and normal esophageal tissues (1/8, 12 5%) for ECNM expression were significant ( P <0 05). The metastatic rate of esophageal cancer with ECNM expression was higher than that with negative ECNM expression (17/18, 94 4% vs 15/23, 65 2%; P <0 05). The difference between human and mouse esophageal cancer tissues for ECNM espression was notsignificant ( P >0 05). CONCLUSION ECNM antibody may be used as a new marker for human esophageal cancer, with different expressions in lymph node metastasis.

关 键 词：食管肿瘤 癌 核基质 免疫学 抗体 免疫组织化学 

分 类 号：R735.103[医药卫生—肿瘤]