纳米脂质体介导p53治疗鼻咽癌的实验研究  

Nanoliposome-mediated p53 gene therapy for nasopharyngeal carcinoma

作　　者：刘海[1] 秦学玲[2] 鲜均明[2] 周光耀[2] 梁传余[2] 

机构地区：[1]川北医学院附属医院耳鼻咽喉科,四川南充637007 [2]四川大学华西医院耳鼻咽喉科,四川成都610041

出　　处：《中国耳鼻咽喉颅底外科杂志》2008年第1期21-24,共4页Chinese Journal of Otorhinolaryngology-skull Base Surgery

摘　　要：目的探讨纳米脂质体介导p53基因在体外转染鼻咽癌细胞株后细胞的生长变化情况。方法不同比例的纳米脂质体与p53质粒的混合物转染鼻咽癌细胞,转染效率最高的组用MTT法和流式细胞术检测鼻咽癌细胞的生长和凋亡情况。结果比例为纳米脂质体/Porf-GFP=2.5/1的转染效率最高,达60%～70%。MTT法测定纳米脂质体包裹p53能明显抑制鼻咽癌细胞株的体外生长,抑制率为67.7%,且转染脂质体/Porf-hp53的鼻咽癌细胞中凋亡细胞占47.8%。结论纳米脂质体作为一种非病毒载体能有效的将p53基因转导入鼻咽癌细胞中,并抑制肿瘤细胞生长,诱发凋亡。Objective To investigate the proliferation of nasopharyngeal carcinoma （NPC） cell by in vitro transfection with nanoliposome encapsulated p 5 3 genes. Methods NPC cells were transfected in vitro with mixtures of nanoliposome and plasmid at different ratios, and the proliferation and apoptosis of transfected cells were detected with MTT assay and flowcytometry respectively in the group with the highest transfection efficiency. Results The transfection efficiency was the highest （6 0 % ～7 0% ） when the ratio of nanoliposome to Porf-GFP was 2. 5. The in vitro growth of transfected cells was significantly inhibited by nanoliposome encapsulated p5 3, with an inhibition rate of 67. 7 %. In flowcytometer detection, 47. 8% of the nanoliposome/Prf-hp53 transfected cells were apoptotic. Conclusion Nanoliposome, as a member of non-viral delivery system, can inhibit tumor growth and induce tumor cell apoptosis by effectively transfecting p53 gene to NPC cells.

关 键 词：纳米脂质体 P53 鼻咽肿瘤 凋亡 转染率 

分 类 号：R739.63[医药卫生—肿瘤]