顺铂对结直肠癌肿瘤浸润淋巴细胞杀伤活性的影响  

The role of CDDP on cytotoxicity of TILs obtained from colorectal tumors

作　　者：许哲[1] 祁小平[1] 刘福坤[1] 黎介寿[1] 

机构地区：[1]南京军区南京总医院全军普外研究所

出　　处：《中华外科杂志》1997年第5期265-267,共3页Chinese Journal of Surgery

摘　　要：为在临床选择有效的免疫化疗方案提供一定的理论依据，作者以结直肠癌肿瘤浸润淋巴细胞（ＴＩＬ）和顺铂（ＣＤＤＰ）为研究对象，对１６名手术治疗的结直肠癌患者，分别观察ＣＤＤＰ体内注射及体外预处理ＴＩＬ和Ｒａｊｉ细胞对ＴＩＬ表面标志和杀伤活性的影响。流式细胞仪检测结果显示，静脉注射ＣＤＤＰ能增加结直肠癌ＴＩＬ中ＣＤ３＋／ＣＤ４＋和ＣＤ３＋／ＣＤ８＋细胞含量，同时增强ＴＩＬ体外杀伤Ｒａｊｉ细胞的活性；而体外以ＣＤＤＰ处理Ｒａｊｉ细胞能增强其对结直肠癌ＴＩＬ杀伤的敏感性。作者认为，对于联合应用ＴＩＬ和ＣＤＤＰ治疗结直肠癌的临床效果有必要进一步研究。The effects of intravenous injection of cisplatin(CDDP) and the incubated with CDDP in vitro on the epitopes and cytotoxicity of TIL obtaind from eight colorectal tumor patients were detected respectively by flow cytometer.There was a significant increase in CD + 3/CD + 8 subset in TIL in patients receiving intravenous injection of CDDP.And the cytotoxicity of these TILs increased significantly.The cytotoxities were positively correlated with the amount of CD + 3/CD + 8 subset in TIL. Raji cells incubated with CDDP in vitro showed increased susceptibility to TIL induced lysis.

关 键 词：结肠肿瘤 直肠肿瘤 药物疗法 顺铂 

分 类 号：R735.305[医药卫生—肿瘤]