肝纤维化血清学诊断指标初探  

Serum targets in auxiliary diagnosis of hepatic fibrosis

作　　者：王伟华[1] 孙克伟[1] 刘伟士[1] 褚裕义[1] 谢凤瑛[1] 邹福菊 谌宁生[1] 刘明德[1] 

机构地区：[1]湖南中医学院第一附属医院肝病研究室

出　　处：《中华医学检验杂志》1997年第3期150-152,共3页

基　　金：国家"八五"攻关课题基金

摘　　要：目的探讨血清Ｎ－乙酰－β－Ｄ氨基葡萄糖苷酶（ＮＡＧ）、腺苷脱氨酶（ＡＤＡ）、羟脯氨酸（Ｈｙｐ）和透明质酸（ＨＡ）含量测定在肝纤维化辅助诊断中的应用价值。方法以四氯化碳（ＣＣｌ４）高脂低蛋白复合因素诱发大鼠肝硬化模型，以分光光度计比色法和放射免疫分析法检测了２０例肝硬化大鼠和９６例各型慢性肝病患者上述指标。结果ＮＡＧ、ＡＤＡ、Ｈｙｐ和ＨＡ含量：在肝硬化大鼠明显高于正常大鼠相应指标（Ｐ＜０．０５或０．０１）；在慢性迁延型肝炎（ＣＰＨ，３５例）、慢性活动型肝炎（ＣＡＨ，３０例）和肝炎后肝硬化（ＬＣ，３１例）组，均显著高于正常对照组（Ｐ＜０．０５或０．００１，但ＣＰＨ组ＡＤＡ除外），且ＬＣ＞ＣＡＨ＞ＣＰＨ。结论ＮＡＧ、ＡＤＡ、Ｈｙｐ和ＨＡ含量测定在肝纤维化辅助诊断中具有良好应用价值。Objective To study the applicable value of acetylglucosaminidase (NAG), adenosine deaminase (ADA), hydroxyproline (Hyp) and hyaluronate (HA) in the detection of auxiliary diagnosis of hepatic fibrosis. Methods The model of rat liver cirrhosis (LC) was induced with a combination of carbon tetrachloride (CC14), high fatty foods, and low protein foods. The four targets listed above came from 20 rats with LC and 96 patients with chronic liver diseases. The targets were then studied using colorimetric analysis with spectrophotometer and radioimmunological analysis. Results In the group of rats with LC, the contents of the four targets were markedly increased compared with the normal group of rats ( P <0.05 or 0.01, respectively). All the above targets in chronic persistant hepatitis (CPH, 35 patients), chronic active hepatitis (CAH, 30 patients) and LC(31 patients) were significantly increased as compared with the healthy donors respectively ( P <0.05 or 0 001), except for the ADA in CPH. And the levels of the targets in LC>in CAH>in CPH. Conclusion All the results above demonstrated that the detection of NAG, ADA, Hyp, and HA contents is of good applicable value in auxiliary diagnosis of hepatic fibrosis.

关 键 词：肝纤维化 血清学诊断 NAG ADA HYP HA 

分 类 号：R575.204[医药卫生—消化系统]