应用基因重组病毒表达产物筛选FBL3小鼠白血病肿瘤特异性抗原表位  

IDENTIFICATION OF A GAG ENCODED CYTOTOXIC T LYMPHCYTE EPITOPE FROM FBL3 LEUKEMIA VIRUS INDUCED TUMORS

作　　者：秦慧莲[1,2] 陈卫[1,2] 陈永良[1,2] 何球藻 

机构地区：[1]上海医科大学免疫学教研室 [2]美国华盛顿大学医学院肿瘤系

出　　处：《中国免疫学杂志》1997年第4期207-210,共4页Chinese Journal of Immunology

基　　金：国家青年自然科学基金

摘　　要：肿瘤特异性抗原是肿瘤免疫研究的中心课题，众多的研究表明Ｔ细胞在肿瘤免疫中起重要作用，因而寻找Ｔ细胞所识别的肿瘤特异性表位并以此为瘤苗的靶抗原有重要的研究价值。以一系列重组Ｆ－ＭｕＬＶｇａｇ病毒载体，用已建立的ＦＢＬ３－ＣＴＬ克隆筛选，并通过Ｈ－２Ｉ类分子结合实验，精确地测得Ｆ－ＭｕＬＶｇａｇ中一个能被为ＦＢＬ３－ＣＴＬ克隆所识别的表位（ＣＣＬＣＬＴＶＦＬ，ｐ８５～９３）。并以此ｐ８５～９３合成肽免疫动物成功诱导出能识别亲本ＦＢＬ３肿瘤细胞。FBL3 is a highly immunogenic murine leukemia of C57BL/6 origin induced by friend murine leukemia virus(MuLV).A series of FBL3 specific CD8 + CTL(FBL3 CTL) clone were generated from C57BL/6 mice immunized by FBL3.By using a series of recombinant vaccinia viruses expressing F MuLV gag gene,the antigenic epitope recognized by FBL3 CTL,was localized to the leader sequence was identified as CCLCLTVFL(positions 85 93) by examining lysis of targets incubated with a series of synthetic peptides.The identity of CCLCLTVFL as the target peptide was validated by showing that immuniztion with the peptide elicited CTL that lysed FBL3.

关 键 词：白血病 肿瘤特异性抗原 CTL 肿瘤免疫学 

分 类 号：R733.703[医药卫生—肿瘤] R730.3[医药卫生—临床医学]