静电纺再生丝素/明胶纳米纤维的结构与性能  

Microstructure and property of electrospun regenerated silk fibroin/gelatin nanofibers

作　　者：鲍韡韡[1] 王曙东[1] 张幼珠[1] 尹桂波[1] 吴佳林[1] 施德兵[2] 董智慧[2] 符伟国[2] 

机构地区：[1]苏州大学材料工程学院,江苏苏州215021 [2]复旦大学附属中山医院,上海200032

出　　处：《纺织学报》2008年第3期1-4,8,共5页Journal of Textile Research

基　　金：江苏省高校重点实验室开放研究课题(S8115033)

摘　　要：以质量分数为98%的甲酸为溶剂,将再生丝素与明胶以质量比70∶30进行共混静电纺丝。研究纺丝液质量分数及乙醇处理对纤维膜的结构及力学、溶解性能等的影响;测定不同纺丝液质量分数及不同厚度下纤维膜的孔隙率及孔径;在纤维膜上进行小鼠成纤维细胞(L929)和人脐静脉内皮细胞(HUVECs)培养实验。结果表明:随着纺丝液质量分数的提高或经乙醇处理后,丝素β化程度提高,纤维结晶度增大,溶失率减小,拉伸强度增大;随着纺丝液质量分数的提高,纤维膜的孔隙率减小,孔径增大,厚度增加时,纤维膜的孔隙率及孔径均减小;L929及HUVECs均能够在纳米纤维膜上黏附、生长和增殖。The electrospinning of regenerated silk fibroin （SF） and gelatin blended with the composition ratio 70：30 was performed with 98% formic acid as spinning solvent. The influence of mass concentration and ethanol treatment on the microstructure, mechanical properties and solubility of electrospun SF/gelatin nanofibers was investigated. The pore diameter and porosity of SF/gelatin fiber membrane with different mass concentrations and different thickness were measured. The mouse fibroblasts and human umbilic vein endothelium cells were seed on SF/gelatin nanofibers scaffold. The results showed that： along with the mass concentrations of spinning solution increasing and ethanol treating, the content of β-sheet of SF in electrospun SF/gelatin nanofibers was increased, crystallinity increased, water-dissolved rate declined, and tensile strength enhanced of electrospun SF/gelatin nanofibers; as the mass concentration of spinning solution increased, the pore diameter of electrospun SF/gelatin nanofibers was decreased and the porosity was increased; both the pore diameter and porosity were decreased with the increase of the thickness of electrospun SF/gelatin nanofibers. The SF/gelatin nanofibers scaffold may be a good candidate for both L929 and HUVECs spreading and proliferating.

关 键 词：再生丝素 明胶 静电纺丝 结构 力学性能 孔隙率 细胞培养 

分 类 号：TS102.512[轻工技术与工程—纺织工程]