存活素反义寡核苷酸对角质形成细胞增殖和凋亡的影响  

Effect of Antisense Oligonucleotides Targeting Survivin on the Proliferation and Apoptosis of Keratinocytes

作　　者：王雷[1] 杨励[1] 吕雅洁[1] 高滢[1] 李巍[1] 高天文[1] 刘玉峰[1] 

机构地区：[1]第四军医大学西京医院全军皮肤病研究所,陕西西安710032

出　　处：《中国皮肤性病学杂志》2008年第3期135-137,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的探讨存活素反义寡核苷酸对角质形成细胞增殖和凋亡的影响。方法以脂质体介导存活素反义寡核苷酸转染体外培养的角质形成细胞。采用MTT方法观察存活素反义寡核苷酸对角质形成细胞生长曲线的影响;采用RT-PCR方法观察存活素反义寡核苷酸对角质形成细胞存活素表达水平的影响;采用流式细胞仪检测存活素反义寡核苷酸对角质形成细胞细胞周期和凋亡的影响。结果存活素反义寡核苷酸作用于角质形成细胞后,细胞增殖受到明显抑制,存活素mRNA表达水平明显下降,细胞出现明显的凋亡现象。结论存活素反义寡核苷核酸能够抑制角质形成细胞增殖,促进角质形成细胞凋亡。提示存活素可能会成为一个新的治疗银屑病的靶分子。Objective To investigate the effect of antisense oligonueleotides targeting survivin on the proliferation and apoptosis of keratinoeytes. Methods The keratinoeytes were transfeeted with antisense oligonueleotides targeting survivin by using liposome. The growth curve of keratinoeytes was detected with MTT eolorimetrie assay ; the effects of antisense oligonueleotides on the mRNA expression of survivin were tested by using RT-PCR;the effects of antisense oligonueieotides on cell cycle and apoptosis of keratinoeytes were tested by using flow eytometry. Results After the tranfeetion with antisense oligonueleotides targeting survivin, the proliferation of keratinoeytes were obviously inhibited, the mRNA expression of survivin was clown regulated, and prominent apoptosis of keratinoeytes was observed. Conclusion Antisense oligonucleotides targeting survivin can inhibit the proliferation and accelerate the apoptosis of keratinoeytes,indicates that survivin may become a new target in treating psoriasis.

关 键 词：存活素 反义寡核苷酸 凋亡 角质形成细胞 银屑病 

分 类 号：R392.31[医药卫生—免疫学]