大肠菌群革兰氏染色技术的实验研究  

Experimental Study on Technique of Gram Staining Coliform Group

作　　者：沈杰[1] 

机构地区：[1]启东市环境监测站,江苏启东226200

出　　处：《干旱环境监测》2008年第1期62-64,共3页Arid Environmental Monitoring

摘　　要：针对影响革兰氏染色正确性的几个因素,通过比较实验,研究了在大肠菌群测定过程中菌龄、细菌密集程度、媒染时间、脱色程度等因素对革兰氏染色正确性的影响,从而得出一最佳革兰氏染色条件组合,并提出了确证革兰氏染色技术操作正确的方法。Using compare- experiment, it was investigated how bacterium age, denseness, mordant stain, decolofize etc. affected the result of Gram stain when we determined coliform group. Furthermore, optimal values of Gram stained were obtained and means of confirming correctness of Gram stain was brought forward.

关 键 词：大肠菌群 革兰氏染色 细胞壁 媒染 脱色 

分 类 号：X830.2[环境科学与工程—环境工程]