五行汤对黑色素瘤小鼠抑瘤作用及免疫功能的影响  

The anti-tumour effect of Wuxing soup and its effect on the immunity of melanoma mice

作　　者：莫非[1] 胡晶莹[2] 甘愉[2] 赵仰星[2] 朱明洁[2] 赵新泰[2] 

机构地区：[1]复旦大学研究生院,上海200032 [2]上海市肿瘤研究所免疫室,上海200032

出　　处：《中国癌症杂志》2008年第3期186-189,共4页China Oncology

摘　　要：背景与目的:民间验方"五行汤"由牛蒡、白萝卜、白萝卜叶、胡萝卜和香菇组成,有一定的抗肿瘤效果,值得深入研究。本研究通过动物体内实验观察五行汤对小鼠黑色素瘤的抑瘤作用和免疫功能的影响,同时检测比较单方和复方作用差异。方法:用不同成分汤剂处理小鼠黑色素瘤细胞B16,MTT法检测汤剂对细胞生长的影响;C57/BL6小鼠接种B16建立小鼠黑色素瘤模型,随机分为阳性组、空白组和各汤剂处理组;接种后第2天给小鼠灌胃,每天1次,25 d后处死小鼠取瘤称重;MTT法检测小鼠T淋巴细胞转化功能,乳酸脱氢酶(LDH)法检测NK细胞杀伤活性。结果:体外实验显示不同成分汤剂均可抑制B16细胞生长,以复方五行汤抑制作用最强,同时呈浓度依赖性;体内实验显示不同成分汤剂有不同程度的抑瘤作用;不同成分汤剂灌胃后小鼠淋巴细胞转化功能相应增强;同时NK细胞杀伤活性也有不同程度的增加;抑瘤作用和免疫功能的单、复方的比较中都是五行汤的作用最强。结论:五行汤对小鼠黑色素瘤有较明确的抑制作用同时可以增强荷瘤小鼠细胞免疫功能。Background aud purpose： Wuxing soup is popular for it＇s anti-cancer effect in folk medicine and deserved to be further studier by modern scientife methods.This research aimed to explore its anti-cancer effect and to study the influence on the immunity of melanoma in mice.Methods：Inhibition of different ingredients and concentrations of Wuxing soup on the growth of the mouse melanoma B16 cells was detected by MTT in vitro.Animal experiment was performed to determine its anti-cancer effect in vivo.C57/B16 mice were randomly divided into three groups after vaccination of B16 cell ,and then given intragastrically with different soups for 25 days.All mice were killed on day 26 after inoculation.The weight of tumors were recorded.The anti-tumor immune function was measured by T lymphocyte transforming assay and NK killing assay.The different effect of different ingredients was also observed.Results：Our result showed that different ingredients soup exerted different inhibition on B16 cell growth and Wuxing soup was the strongest one of all and in dose-dependent manner in vitro.The animal experiment indicated that different ingredients soup has different inhibition on melanoma.the soup-treated mouse display improved T lymphocyte transforming assay and NK killing ability.Among the different soups,Wuxing soup showed the strongest anti-cancer effect and immune enhancement .Conclusions：Wuxing soup is an effective anti-cancer agent in melanoma mice and can enhance the immunity of the mice with melanoma.

关 键 词：五行汤 抑瘤作用 免疫增强作用 培养的肿瘤细胞 小鼠 

分 类 号：R73-36[医药卫生—肿瘤]