脑缺血-再灌注损伤白细胞渗出和L-选择素表达及人工合成E-选择素的干预作用  

Leukocyte Infiltration and Expression of L-selectin after Cerebral Ischemic Reperfusion and the Protective Effects of Artificial Synthetic E-selectin in Rats

作　　者：黄亚波[1] 张世明[1] 

机构地区：[1]苏州大学附属第一医院神经外科,215007

出　　处：《中国临床神经科学》2008年第2期149-152,共4页Chinese Journal of Clinical Neurosciences

摘　　要：目的:研究人工合成E-选择素对脑缺血再灌注后白细胞渗出和L-选择素表达的影响。方法:应用线栓法建立局灶性脑缺血再灌注大鼠模型,采用流式细胞术检测脑缺血再灌注后白细胞渗出和L-选择素表达。结果:L-选择素在脑缺血再灌注2h后已经表达,再灌注6h达高峰;在再灌注12h后CD45表达逐渐增加。应用人工合成E-选择素后,L-选择素表达在各时间点均下降(P<0.05);在再灌注12、24和48h时间点CD45的表达明显低于对照组(P<0.05)。结论:人工合成E-选择素能够明显抑制L-选择素的表达,使白细胞的渗出明显减少,对大鼠脑缺血再灌注损伤起到保护作用。Aim： To investigate the effects of artificial synthetic E-selectin on the leukocyte infiltration and expression of L-selectin after cerebral ischemic reperfusion in rats. Methods： The model of focal cerebral ischemia in rats were established by suture-occluded method. Leukocyte infiltration and expression of L- selectin after cerebral ischemic reperfusion were tested by flow cytometry. Results： After cerebral ischemic reperfusion, L-selectin is detected as early as 2 h, peak at 6h; CD45 gradually increase after 12 h reperfusion. After treated with applying artificial synthetic E-selectin,the decrease of L-selectin is comparatively lower than the focal cerebral ischemia group at various times（P〈0.05）, the decrease of CD45 is comparatively lower than the focal cerebral ischemia group at 12 h, 24 h and 48 h（P〈0.05）. Conclusion ： The artificial synthetic E-selectin can significantly inhibit expressions of L-selectin and may reduce leukocyte infiltration to protect against cerebral ischemia reperfusion injury.

关 键 词：脑缺血 白细胞渗出 L-选择素 人工合成E-选择素 流式细胞术 

分 类 号：R743[医药卫生—神经病学与精神病学]