低剂量辐射诱导体外EL-4淋巴瘤细胞周期进程的适应性反应  

Adaptive Response of EL-4 Lymphoma Cell Cycle Progression Induced by Low Dose Radiation in Vitro.

作　　者：刘淑春[1] 王志成[1] 刘扬[1] 张萱[1] 龚守良[1] 

机构地区：[1]吉林大学公共卫生学院卫生部放射生物学重点实验室,吉林长春130021

出　　处：《中国辐射卫生》2008年第1期3-4,共2页Chinese Journal of Radiological Health

摘　　要：目的观察低剂量辐射诱导EL-4淋巴瘤细胞周期进程的适应性反应。方法用X射线照射离体EL-4淋巴瘤细胞，其诱导剂量（D1）为25～200mGy（12．5mGy／min），攻击剂量（D2）为1．5Cy（287mGy／min），D1和D2间隔6h。通过流式细胞仪检测其细胞周期各时相百分数的变化。结果当D1和D2分别为25～100mGy和1．5Gy，或分别为75mGy和0．5～2．0Gy，D1＋D2各组G0／G1期细胞百分数不同程度低于各自D2组，而S期细胞百分数明显高于各自D2组（P〈0．05、P〈0．01或P〈0．001）。结论上述结果显示，EL-4淋巴瘤细胞在1．0～2．0Gy照射前6h接受25～100mGy照射，可在体外诱导其细胞周期进程的适应性反应。Objective To explore the adaptive response of EL -4 cycle progression induced by low dose radiation in vitro. Methods EL - 4 lymphoma cells were irradiated with the inductive doses （ D1：25 - 200 mcy, 12.5 mGy/min） and the challenging dose （ D2 ： 0.5 - 3.0 Gy, 287 mGy/min）. The interval time between D1 and D2 was 6 h. The cell percentages of each cell cycle phase were measured with flow eytometry. Results The cell percentages of G0/G1 phase in the D1 ＋ D2 group with25 - 100 mGy （ D1 ） and 1.5 Gy （D2） or 75 mGy （ D1 ） and 25 - 200 mGy （D2） were invaribly lower than those in the D2 group, while the cell percentages of S phase increased significantly （P 〈 0.05, P 〈 0. 01 or P 〈 0. 001 ）. Conclusion The results suggest that EL -4 lymphoma cells were irradiated with 25 - 100 mGy at 6h before 1.0 -2.0 Gy exposure. The low - dose radiation could induce the adaptive response of the cell cycle progression in vitro.

关 键 词：低剂量辐射 淋巴瘤细胞 细胞周期 适应性反应 

分 类 号：R730.55[医药卫生—肿瘤] R811.5[医药卫生—临床医学]