荧光素酶活体发光技术在肺癌分子影像学研究中的初步应用  

Preliminary Experiment with Lung Cancer Cell Expressing Luciferase Reporter for in Vivo Molecular Imaging

作　　者：王建东[1] 张帆[1] 黄惠莲[2] 藏风超[3] 赵艳娥[1] 张禄卿[4] 李朝军[2] 田捷[5] 滕皋军[3] 何玉龙[4] 周晓军[6] 卢光明[1] 

机构地区：[1]南京军区南京总医院医学影像科,210002 [2]南京师范大学 [3]东南大学中大医院医学影像科 [4]南京大学模式动物研究所 [5]中国科学院自动化研究所 [6]南京军区南京总医院病理科,210002

出　　处：《临床放射学杂志》2008年第2期270-272,共3页Journal of Clinical Radiology

基　　金：国家基础研究项目973资助(编号:2006,CB705707)

摘　　要：目的建立稳定表达荧光素酶报告基因的人肺癌细胞系,并将该肺癌细胞接种于严重联合免疫缺陷(SCID)小鼠的皮下,构建异种移植动物模型,为下一步进行肺癌进展、转移以及药物敏感性等相关的分子影像学研究奠定基础。材料与方法利用表达重组荧光素酶基因的慢病毒载体(Lenti-Luc)感染人肺癌细胞系NCI-H460,获得稳定表达荧光素酶基因的肺癌细胞,将该细胞接种于SCID小鼠的皮下。肿瘤细胞接种2周后,利用生物发光成像系统及MRI对肿瘤进行检测,确定肿瘤的大小和位置,并进行组织病理学检查验证。结果接种肺癌细胞的4只SCID小鼠中,均生长出肿瘤。生物发光检测和MRI的结果相符,病理证实小鼠皮下肿块为癌组织。结论成功建立了稳定表达荧光素酶报告基因的肺癌细胞系及异种移植动物模型。荧光素酶活体发光是一种进行肿瘤分子影像学研究的重要手段。ObjectiveTo establish a human lung cancer model with stable expression of luciferase for in vivo imaging, in order to perform extensive studies on progression, metastasis and drug sensitivity of lung cancer. Materials and Methods The human lung cancer cell line NCI-H460 was transduced with the Lenti Luc viruses and tumor cells with stable expression of luciferase were established by the limited dilution. The tumor cells were then subcutaneously implanted into SCID mice. Two weeks after implantation, tumor images were acquired using the IVIS Imaging System and Magneitc Resonance Imaging. The tumor tissues were also processed for further histological analysis. Results Primary tumors were detected in all the implanted mice. The imaging data from both IVIS and MRI systems were consistent. Tissues were pathologically confirmed as cancers. Conclusion We have successfully established a human lung tumor model with stable expression of luciferase, which are useful for the lung cancer study in the future.

关 键 词：荧光素酶 生物发光分析 肺癌 分子影像学 

分 类 号：R734.2[医药卫生—肿瘤]