ERK在慢性哮喘大鼠气道平滑肌细胞增殖中的作用  

Role of ERK on proliferation of airway smooth muscle cells in chronic asthmatic rats

作　　者：白晶[1] 刘先胜[1] 徐永健[1] 谢敏[1] 倪望[1] 陈士新 

机构地区：[1]华中科技大学同济医学院附属同济医院呼吸内科,湖北武汉430030

出　　处：《中国病理生理杂志》2008年第3期417-422,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30400195);武汉市青年科技晨光计划资助项目(No.20045006071-8)

摘　　要：目的:观察细胞外信号调节蛋白激酶(ERK)在慢性哮喘大鼠气道平滑肌细胞的表达,以探讨ERK信号通路在气道平滑肌增殖中的作用。方法:病理图像分析慢性哮喘大鼠气道重塑,免疫组化法检测ERK和PC-NA在肺内表达,激光共聚焦显微镜分析ERK1/2、磷酸化ERK1/2和PCNA在气道平滑肌的共表达,免疫印迹和原位杂交检测气道平滑肌中ERK和PCNA蛋白以及mRNA的表达。结果:慢性哮喘大鼠有气道平滑肌层增厚,出现结构重塑。ERK和PCNA在肺内表达增强,同时在气道平滑肌上有ERK和PCNA蛋白与mRNA表达增加。结论:ERK可能是介导慢性哮喘气道重建中平滑肌增殖的重要信号通路之一。AIM： To investigate the roles of extracellular signal -regulated kinase（ERK） signaling pathway on regulating proliferation of airway smooth muscle by observing the expression of ERK in airway smooth muscle （ASM） in chronic asthmatic rats. METHODS ： Airway remodeling was detected in chronic asthmatic rats by using image analysis system. The expressions of ERK and proliferating cell nuclear antigen（PCNA） in lung tissue from chronic asthmatic rats were observed by immuocytochemistry staining. The expressions of ERK1/2, p-ERK1/2 and PCNA were detected in airway smooth muscle （ASM） by immunofluorescence double staining with confocal microscopy, and the expressions of protein or mRNA of ERK and PCNA in ASM were also detected by immunoblotting and hybridization in situ,respectively. RESULTS： The thickening of smooth muscle and structural remodeling in airway were observed in chronic asthmatic rats by image analysis. The enhanced expressions of ERK and PCNA appeared obviously increased in same lung tissue and the expressions of protein or mRNA of ERK and PCNA were significantly increased in ASM. CONCLUSION： ERK signal pathway might be an important pathway on regulating cell proliferation of ASM resulting in asthmatic airway remodeling.

关 键 词：哮喘 有丝分裂素激活蛋白激酶类 气道平滑肌细胞 增殖细胞核抗原 

分 类 号：R363[医药卫生—病理学]