DIM增强顺铂抑制PC-3细胞增殖及诱导凋亡的效应  

DIM enhances effect of cis-diamminedichloroplatinum on growth inhibition and apoptosis in human prostate cancer cell PC-3

作　　者：赵燕颖[1] 秦中国[1] 邱伟智[1] 潘玉琢[1] 赵丽娟[1] 赵雪俭[1] 

机构地区：[1]吉林大学基础医学院病理生理学教研室,吉林长春130021

出　　处：《中国病理生理杂志》2008年第3期490-493,共4页Chinese Journal of Pathophysiology

基　　金：中日政府间专项技术合作项目资助(No.59项);科技部国际科技合作重点资助项目(No.2004DFB02000)

摘　　要：目的:观察顺铂(DDP)和3,3-二吲哚基甲烷(3,3-diindolylmethane,DIM)联合应用对人前列腺癌PC-3细胞增殖和凋亡的影响。方法:采用MTT法检测PC-3细胞增殖抑制情况,用流式细胞术及吖啶橙染色法分析细胞凋亡的变化,用RT-PCR检测抑癌基因p21的表达变化。结果:60μmol.L-1的DIM与0.4 mg.L-1的DDP联合可有效抑制PC-3细胞增殖并诱导其凋亡,其效果与单用4 mg.L-1DDP相同。DIM与DDP联合试验中细胞生长抑制率和凋亡率也明显高于单用DIM处理组(P<0.05)。RT-PCR结果表明:单用DIM和DIM与DDP联合用药都能明显增强p21基因的表达,但联合用药的效果更明显。结论:DIM能显著增强DDP对PC-3细胞的增殖抑制和诱导凋亡的效应。AIM ： To study the effects of the combination of cis - diamminedichloroplatinum （DDP） and 3, 3 - diindolylmethane （DIM） on the growth and apoptosis of human prostate cancer cell PC - 3. METHODS： MTT method was applied to detect the cell growth inhibitory rate. The cell apoptosis was measured by the flow cytometry and acridine orange staining method. The expression of the anti - oncogene p21 was detected by RT - PCR technique. RESULTS： The combination of 60 μmol·L^-1 DIM and 0.4 mg·L^-1 DDP effectively inhibited the growth and induced apoptosis in PC - 3 cells. This result was the same as the effect of using 4 mg·L^- 1 DDP only. The cell growth inhibitory and apoptosis rates for the combination of DIM and DDP were much higher than those for the individual effect. Both the combination and the single effect of these two medicines （ i. e. , DIM and DDP） all strengthen p21 mRNA expression significantly, and the effect of combination was more significant. CONCLUSION： DIM significantly enhances the effects of DDP on the growth inhibition and apoptosis induction in PC -3 cells.

关 键 词：细胞凋亡 二吲哚基甲烷 顺铂 前列腺肿瘤 

分 类 号：R363[医药卫生—病理学]