RNAi干扰食管癌EC9706细胞MTA1基因表达对侵袭和迁移的影响  

RNAi silencing MTA1 gene inhibits invasion and migration of esophageal carcinoma 9706 cells

作　　者：杨松华[1] 赵国强[1] 郑红[1] 赵继敏[1] 董子明[1] 

机构地区：[1]郑州大学基础医学院病理生理教研室,郑州450001

出　　处：《第三军医大学学报》2008年第6期503-505,共3页Journal of Third Military Medical University

基　　金：教育部"十五"211工程重点学科建设项目(教重办2002第2号)~~

摘　　要：目的采用RNAi抑制食管癌EC9706细胞株MTA1基因的表达,观察对食管癌细胞侵袭和迁移的影响。方法用脂质体包裹转染沉默MTA1表达的siRNA表达载体入食管癌细胞EC9706。定量RT-PCR和免疫印迹分别检测MTA1 mRNA和蛋白表达情况;划痕损伤实验和细胞体外侵袭实验分别测定迁移和侵袭的影响。结果定量RT-PCR检测显示,MTA1基因的表达水平明显降低;转染沉默MTA1的siRNA表达载体组的食管癌细胞划痕未愈合,穿透Matrigel的细胞数量明显减少,与未转染组、转染空载体组和转染无关序列siRNA载体组比较差异有显著性(P<0·05)。结论RNA干扰介导的MTA1基因表达沉寂可有效降低食管癌细胞侵袭和迁移;MTA1基因可能成为肿瘤治疗上有前途的分子靶点。Objective To observe the effect of RNAi that silences MTA1 gene on invasion and migration of esophageal carcinoma 9706 cells. Methods The siRNA expression vector that silences MTA1 gene was transfected into EC9706 cells by liposome. MTAI mRNA and protein expressions were detected through quantitative RT-PCR and Western blot, respectively. The invasion and migration of EC9706 cells were evaluated by scrape wound healing assay and cell invasion assay in vitro. Results MTA1 gene expression significantly decreased. The scrape wound of EC9706 cells healed more slowly and the cell population that cut through Matrigel were less in the EC9706 cells transfected with siRNA expression vector than non-transfected EC9706 cells and the EC9706 cells transfected with blank vector （P 〈 0. 05 ）. Conclusion RNAi silencing MTA1 gene inhibits the invasion and migration of esophageal carcinoma effectively. It is supposed that MTA1 gene may be a prospective molecule target in tumor therapy.

关 键 词：MTA1 RNA干扰 侵袭 迁移 

分 类 号：R394.3[医药卫生—医学遗传学] R73-37[医药卫生—基础医学]