雌激素受体β蛋白及其同分异构体mRNA在女性慢传输性便秘患者乙状结肠壁内的表达  

Expression of ERβ and its isoform in sigmoid colon of female patients with slow transit constipation

作　　者：张广军[1] 刘宝华[1] 童卫东[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所普通外科,重庆400042

出　　处：《第三军医大学学报》2008年第6期543-545,共3页Journal of Third Military Medical University

基　　金：重庆市自然科学基金(2006BB5114)~~

摘　　要：目的研究雌激素受体β(estrogen receptor β,ERβ)蛋白及其同分异构体mRNA在女性慢传输性便秘(slow transit constipation,STC)患者乙状结肠的表达。方法采用RT-PCR及Western blot技术检测20例女性STC患者以及20对照组乙状结肠ERβ蛋白及其同分异构mRNA的表达。结果ERβ1、ERβ2及ERβ5 mRNA在女性STC患者及对照组乙状结肠均有表达,ERβ3及ERβ4 mRNA在两组中均无表达,STC组ERβ1、ERβ2及ERβ5 mRNA表达量均低于对照组,差异有统计学意义(P<0·01)。Western blot结果显示STC组乙状结肠ERβ蛋白表达较对照组明显降低,差异有统计学意义(P<0·01)。结论ERβ与STC的发病有关。Objective To investigate the expressions of ERβ protein and ERβ isoform mRNA in sigmoid colon of female slow transit constipation patients. Methods RT-PCR and Western blot were used to detect the expression of ERβ protein and ERβ isoform mRNA of 20 patients with STC and 20 age-matched controls. Resuits ERβ protein and ERβ1, ERβ2 and ERβ5 mRNA were detected in the two groups lower in STC group than that in control group （P 〈 0.01 ）, but ERβ3 and ERβ4 mRNA were not detectable. Conclusion ERβ is involved in genesis of STC.

关 键 词：慢传输性便秘 雌激素受体Β 同分异构体 

分 类 号：R322.45[医药卫生—人体解剖和组织胚胎学] R394.2[医药卫生—基础医学]