S100A9 Galectin7在同时放化疗敏感性不同宫颈癌中的表达及意义  

The Expression of S100A9 and Galectin7 in Cervical Carcinoma Tissues with Different Concurrent Chemoradiotherapy Sensitivity

作　　者：朱红[1] 曾亮[2] 涂青松[1] 肖自强[3] 

机构地区：[1]中南大学湘雅医院肿瘤科,长沙市410008 [2]湖南省肿瘤医院病理科 [3]中南大学湘雅医院卫生部蛋白质组重点实验室

出　　处：《中国肿瘤临床》2008年第5期277-280,289,共5页Chinese Journal of Clinical Oncology

基　　金：湖南省自然科学基金项目(编号:06JJ4199);长沙市科委科研基金资助(编号:K06ST006-32)

摘　　要：目的:观察S100A9和Galectin7在同时放化疗敏感性不同宫颈癌组织中的表达及与宫颈癌同时放化疗敏感性的关系。方法:收集进行治疗前中晚期宫颈癌组织标本,病理诊断均为中分化鳞癌,放化疗后根据WHO实体瘤疗效判断标准,将收集的宫颈癌组织标本分为高敏感组和低敏感组。提取组织总蛋白,进行双向凝胶电泳和质谱分析鉴定差异表达蛋白,分别应用Westernblot和免疫组织化学方法检测组织中蛋白的表达。结果:筛选得到在同时放化疗敏感性不同宫颈癌组织中差异表达蛋白S100A9和Galectin7,它们在高敏感组中的表达均高于低敏感组。WesternBlot检测结果与蛋白质组学结果一致。免疫组化结果显示S100A9在高敏感组中的表达强度和表达率(88.3%,53/60)均显著高于低敏感组(28.6%,10/35),Galectin7在高敏感组中的表达强度及表达率(91.7%,55/60)显著高于低敏感组(40%,14/35),差异均有统计学意义。结论:S100A9和Galectin7的表达与宫颈癌同时放化疗敏感性有关,可能作为同时放化疗敏感性预测的标志物。Objective： To observe the expression of S100A9 and Galectin7 in cervical carcinoma tissues with different concurrent chemoradiotherapy sensitivity and to analyze its significance. Methods： The fresh carcinoma tissues were collected from 10 untreated cervical carcinoma patients. According to the response to concurrent chemoradiotherapy, the tissues were classified into two groups： the high sensitivity group（HS） and the low sensitivity group（LS）. Protein separation was performed by two-dimensional gel electrophoresis （2-DE）. The peptide mass fingerprintings （PMF） were acquired by matrix assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF-MS） and the proteins were identified by data searching in the Mascot-database. The differentially expressed proteins were assayed by Western blot and immnnohistoehemistry. Results： Most of the gels were clear and successfully and reproductively analyzed. It showed that the expression of S100A9 and Galectin7 was higher in the HS group than in the LS group. Conclusion： The expression of S100A9 and Galectin7 was associated with concurrent ehemoradiotherapy sensitivity in cervical carcinoma.

关 键 词：宫颈癌 同时化放疗 蛋白质组学 免疫组织化学 免疫印迹 

分 类 号：R737.33[医药卫生—肿瘤]